CORRELATION BETWEEN SYNTHESIS OF HEAT-SHOCK PROTEINS AND DEVELOPMENT OF THERMOTOLERANCE IN CHINESE-HAMSTER FIBROBLASTS
CORRELATION BETWEEN SYNTHESIS OF HEAT-SHOCK PROTEINS AND DEVELOPMENT OF THERMOTOLERANCE IN CHINESE-HAMSTER FIBROBLASTS
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DOI：
10.1073/pnas.79.10.3218
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
WERB, Z
中科院分区：
文献类型：
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作者：
LI, GC;WERB, Z
细胞中响应各种环境应激而诱导或增强了称为“热休克”蛋白的蛋白质家族的合成，这表明这些蛋白质可能执行细胞生存所必需的功能。由于短暂的非致命热处理可显着诱导对随后的致命热处理（耐热性）的短暂抵抗，因此我们研究了热处理（41-46 摄氏度）对平台期中国仓鼠成纤维细胞 (HA-1) 细胞中蛋白质合成和细胞存活的影响。经过显着抑制总蛋白合成（46 摄氏度）或不抑制总蛋白合成（41 摄氏度）的热处理后，热休克蛋白的合成比未加热的细胞大大增强，并且当细胞受到随后的致死热处理的攻击时，细胞存活率增加了 10(2) 至 10(6) 倍。热休克蛋白的合成与耐热性的发展密切相关，并且这些蛋白的稳定性与长达 36 小时的耐热性持续存在良好相关。亚砷酸钠、缺氧和乙醇也诱导热休克蛋白的合成和瞬时耐热性。对单个蛋白质的定性分析表明，Mr 70,000 或 87,000 的多肽的合成和持久性最符合耐热性动力学。
Synthesis of a family of proteins called "heat shock" proteins is induced or enhanced in cells in response to various environmental stresses, suggesting that these proteins may perform functions essential to cell survival. Because a brief, nonlethal heat treatment can dramatically induce a transient resistance to a subsequent lethal heat treatment (thermotolerance), we examined the effect of heat treatment (41-46 degrees C) on protein synthesis and cell survival in plateau-phase Chinese hamster fibroblast (HA-1) cells. After heat treatments that either drastically inhibited total protein synthesis (46 degrees C) or did not suppress it (41 degrees C), the synthesis of heat shock proteins was greatly enhanced over that in unheated cells, and cell survival was increased 10(2)- to 10(6)-fold when cells were challenged by a subsequent lethal heat treatment. The synthesis of heat shock proteins correlated well with the development of thermotolerance, and the stability of these proteins correlated well with the persistence of thermotolerance up to 36 hr. Sodium arsenite, hypoxia, and ethanol also induced both the synthesis of heat shock proteins and transient thermotolerance. A qualitative analysis of individual proteins suggests that the synthesis and persistence of polypeptides of Mr 70,000 or 87,000 most closely conformed to the kinetics of thermotolerance.