MODULATION OF PROESTROUS SURGE OF LUTEINIZING-HORMONE BY ELECTROCHEMICAL STIMULATION OF AMYGDALA AND HIPPOCAMPUS IN UNANESTHETIZED RAT
MODULATION OF PROESTROUS SURGE OF LUTEINIZING-HORMONE BY ELECTROCHEMICAL STIMULATION OF AMYGDALA AND HIPPOCAMPUS IN UNANESTHETIZED RAT
复制标题
DOI：
10.1016/0006-8993(77)90237-2
复制
发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
SAWYER, CH
中科院分区：
文献类型：
--
作者：
CARRILLO, AJ;RABII, J;SAWYER, CH
在自由活动大鼠模型上，研究了杏仁核和海马区在控制垂体促黄体生成素(LH)释放中的作用。将单极不锈钢电极植入雌性大鼠杏仁皮质内侧(CM)、杏仁基底外侧(BL)和背侧海马区。动物术后恢复，连续发情4天后，在发情间期下午插入慢性心房插管，第二天(发情前期)对双侧杏仁核或海马区进行电化学刺激(20~50个阳极DC120个S)，12~21个小时每隔90分钟采血一次，进行黄体生成素放射免疫分析。第二天，检查输卵管是否有排卵，以此作为排卵的证据。杏仁核内皮细胞对促黄体生成素的释放有两种不同的影响。在临界期(14.00-16.00h)前的13.45h刺激杏仁核背核能有效地延缓和减少LH峰，而在12.00h刺激杏仁中央核则使LH峰的时间提前同步。与背侧海马区应用ECS的结果相反，两组大鼠均排卵，9只大鼠中有7只完全阻断了黄体生成素的峰和排卵。在自由活动的大鼠中，海马体对黄体生成素的释放有很强的抑制作用，而杏仁核在这一过程中起着调节作用。
The roles played by the amygdala and hippocampus in controlling the release of pituitary luteinizing hormone (LH) were studied in the freely moving rat. Monopolar stainless steel electrodes were implanted into the corticomedial (CM) amygdala, basolateral (BL) amygdala and dorsal hippocampus of female rats. When the animal had recovered from surgery and had shown 2 consecutive 4 day estrous cycles, a chronic atrial cannula was introduced during the afternoon of diestrus II. On the following day (proestrus) electrochemical stimulation (ECS) was applied (20-50 .mu.A anodal DC 120 s) bilaterally to the amygdala or hippocampus, and blood samples were taken every 90 min from 12.00-21.00 h for radioimmunoassay (RIA) of LH. Next day, uterine tubes were examined for ova as evidence of ovulation. ECS of the amygdala exerted 2 divergent influences on LH release. Stimulation of the BL amygdala at 13.45 h, just before the critical period (14.00-16.00 h), was effective in delaying and reducing the LH surge, whereas ECS of the CM amygdala at 12.00 h resulted in an early synchronization in the timing of the LH curves. All of the rats in both groups ovulated, in contrast to the results of applying ECS to the dorsal hippocampus; there the LH surge and ovulation were completely blocked in 7 of 9 rats. In the freely moving rat, the hippocampus can exert a potent inhibitory influence on LH release whereas the amygdala plays a modulatory role in the process.