INFLUENCE OF THE VITAMIN-D-BINDING PROTEIN ON THE SERUM CONCENTRATION OF 1,25-DIHYDROXYVITAMIN-D3 - SIGNIFICANCE OF THE FREE 1,25-DIHYDROXYVITAMIN-D3 CONCENTRATION
INFLUENCE OF THE VITAMIN-D-BINDING PROTEIN ON THE SERUM CONCENTRATION OF 1,25-DIHYDROXYVITAMIN-D3 - SIGNIFICANCE OF THE FREE 1,25-DIHYDROXYVITAMIN-D3 CONCENTRATION
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DOI：
10.1172/jci110072
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发表时间：
1981-01-01
影响因子：
15.9
通讯作者：
DEMOOR, P
中科院分区：
文献类型：
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作者：
BOUILLON, R;VANASSCHE, FA;DEMOOR, P
研究了维生素D及其代谢产物的血清结合蛋白(DBP)对其主要配体25-羟基维生素D3(25-OHD3)和1，25-二羟基维生素D3(1，25-(OH)2D3)浓度的影响。1，25-(OH)2D3和DBP在正常女性受试者中的浓度(45。+-。14 ng/L和333.+-。58毫克/L，平均+-。SD；n=58)在服用ESTRO-孕激素(69。+-)期间增加。27 ng/L和488.+-。分别为90 mg/L(n=29)，而25-OHD3浓度不变。这些女性的1，25-(OH)2D3浓度与DBP浓度呈正相关。在妊娠结束时，1，25-(OH)2D3的总浓度(97.+-.26 ng/L，n=40)和舒张压(616±-)。84 mg/L)均显著高于非妊娠女性和配对脐带血样本(48.5+-)。11 ng/L和2 66.+-。41 mg/L)。在怀孕女性及其婴儿中，25-OHD3有明显的季节变化，而在同一样本中，DBP和1，25-(OH)2D3的浓度全年保持不变。服用ESTRO孕激素的妇女体内的游离1，25-(OH)2D3指数，即类固醇与DBP的摩尔比，仍然保持正常，这可能解释了尽管1，25-(OH)2D3总浓度很高，但她们肠道钙吸收正常。在妊娠期间，游离的1，25(OH)2D3指数在妊娠35wk之前保持正常，但在妊娠的最后几周，两个循环中的游离1，25-(OH)2D3指数增加。在妊娠35周和40周时，母血和脐血中(总的和游离的)25-OHD3和1，25-(OH)2D3浓度之间存在高度显著的相关性。
The influence of the serum binding protein (DBP) for vitamin D and its metabolites on the concentration of its main ligands, 25-hydroxyvitamin D3 (25-OHD3) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) was studied. The concentration of both 1,25-(OH)2D3 and DBP in normal female subjects (45 .+-. 14 ng/l and 333 .+-. 58 mg/l, mean .+-. SD, respectively; n = 58) increased during the intake of estro-progestogens (69 .+-. 27 ng/l and 488 .+-. 90 mg/l, respectively; n = 29), whereas the 25-OHD3 concentration remained unchanged. A positive correlation was found between the concentrations of 1,25-(OH)2D3 and DBP in these women. At the end of pregnancy, the total concentrations of 1,25-(OH)2D3 (97 .+-. 26 ng/l, n = 40) and DBP (616 .+-. 84 mg/l) are both significantly higher than in nonpregnant females and paired cord serum samples (48 .+-. 11 ng/l and 266 .+-. 41 mg/l, respectively). A marked seasonal variation of 25-OHD3 was observed in pregnant females and their infants, whereas in the same samples the concentrations of both DBP and 1,25-(OH)2D3 remained constant throughout the year. The free 1,25-(OH)2D3 index, calculated as the molar ratio of this steroid and DBP, remains normal in women taking estro-progestogens and this might explain their normal intestinal Ca absorption despite a high total 1,25-(OH)2D3 concentration. In pregnancy the free 1,25(OH)2D3 index remains normal up to 35 wk of gestation, but during the last weeks of gestation, the free 1,25-(OH)2D3 index increases in both circulations. A highly significant correlation exists between the (total and free) 25-OHD3 and 1,25-(OH)2D3 concentrations in maternal and cord serum both at 35 and 40 wk of gestation.