Cloning and expression of the cDNA encoding rat tissue inhibitor of metalloproteinase 3 (TIMP-3).
Cloning and expression of the cDNA encoding rat tissue inhibitor of metalloproteinase 3 (TIMP-3).
复制标题
编码大鼠组织金属蛋白酶抑制剂 3 (TIMP-3) 的 cDNA 的克隆和表达。
DOI：
10.1016/0378-1119(95)00783-0
复制
发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Moses,MA
中科院分区：
文献类型：
--
作者：
Wu,I;Moses,MA
我们用PCR克隆法克隆了大鼠金属蛋白酶组织抑制剂3（TIMP-3）的cDNA。序列分析揭示了含有211个氨基酸的开放阅读框，其分别显示与小鼠TIMP-3的99%同一性和与人TIMP-3的95%同一性。TIMP-3在大鼠肾、肺和心脏中呈高水平表达。
We have cloned the cDNA encoding the rat tissue inhibitor of metalloproteinase 3 (TIMP-3) by a PCR cloning method. Sequence analysis reveals an open reading frame containing 211 amino acids that show 99% identity to mouse TIMP-3 and 95% identity to human TIMP-3, respectively. High-level expression of TIMP-3 was detected in rat kidney, lungs and heart.