MEASUREMENT OF CIRCULATING CORTICOTROPIN-RELEASING FACTOR IN MAN
MEASUREMENT OF CIRCULATING CORTICOTROPIN-RELEASING FACTOR IN MAN
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DOI：
10.1677/joe.0.1130123
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发表时间：
1987-04-01
影响因子：
4
通讯作者：
REES, LH
中科院分区：
文献类型：
--
作者：
CUNNAH, D;JESSOP, DS;REES, LH
利用Vycor玻璃，建立了一种放射免疫测定法，用于测定从人血浆中提取的促肾上腺皮质激素释放因子（CRF-41）。检测灵敏度为20 ng/l，组内和组间变异系数分别为10.2%和11.4%。血浆CRF-41正常范围< 20 ~ 110 ng/l （n=46）。库欣病、尼尔森综合征和艾迪生病患者血浆中CRF-41浓度均在正常范围内。在这些综合征中，CRF-41与ACTH没有相关性。2例异位ACTH综合征患者血浆中CRF-41浓度升高。在正常受试者中，胰岛素诱导的低血糖、地塞米松治疗或喂养后血浆CRF-41没有变化，CRF-41浓度的变化不反映血浆皮质醇浓度的昼夜变化。妊娠晚期妇女血浆中免疫反应性CRF浓度升高（550-9300 ng/l），凝胶过滤层析显示这包括CRF-41和更高分子量的形式。反相高效液相色谱法还发现血浆和胎盘提取物中存在多个免疫反应性CRF峰。
A radioimmunoassay was developed to measure corticotrophin-releasing factor (CRF-41) extracted from human plasma using Vycor glass. Assay sensitivity was 20 ng/l and intra- and interassay coefficients of variation were 10.2 and 11.4% respectively. The normal range of plasma CRF-41 was < 20-110 ng/l (n=46). Plasma concentrations of CRF-41 in patients with Cushing''s disease, Nelson''s syndrome and Addison''s disease were within the normal range. No correlation was found between CRF-41 and ACTH in these syndromes. Two patients with the ectopic ACTH syndrome had increased plasma concentrations of CRF-41. In normal subjects no changes in plasma CRF-41 occurred after insulin-induced hypoglycaemia, treatment with dexamethasone or feeding, and changes in the concentrations of CRF-41 did not reflect circadian changes in plasma concentrations of cortisol. Concentrations of immunoreactive CRF in plasma of women in the third trimester of pregnancy were increased (550-9300 ng/l) and gel filtration chromatography showed that this comprised CRF-41 and a higher molecular weight form. Reversed phase high-performance liquid chromatography also revealed multiple peaks of immunoreactive CRF in extracts of plasma and placenta.