IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells.
IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells.
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DOI：
10.4049/jimmunol.162.12.7198
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发表时间：
1999-06
影响因子：
4.4
通讯作者：
A. Oliver;F. Martin;J. Kearney
中科院分区：
文献类型：
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作者：
A. Oliver;F. Martin;J. Kearney
Ag相遇将从脾中的成熟B淋巴细胞池中募集Ag特异性细胞，并激活它们以执行效应子功能：产生Ab形成细胞（浆细胞）并将Ag呈递给T细胞。我们比较了成熟卵泡和边缘区细胞发育成效应B细胞的能力。边缘区B细胞的产生及其在边缘窦区的定位不依赖于T细胞和CD 40配体，这表明它们不代表一个生殖后中心群体。与成熟的再循环卵泡B细胞相比，它们表达先前抗原经历的几个特征，包括新鲜分离和体外刺激后较高水平的B7.1（CD 80）和B7.2（CD 86）。在用LPS或抗CD 40 Ab体外短暂刺激6- 8小时后，边缘区B细胞成为有效的APC。此外，它们响应于低剂量的T非依赖性多克隆刺激（LPS）而增殖和分化成浆细胞的能力远大于滤泡B细胞。这些发现表明脾成熟B淋巴细胞内的功能异质性，边缘区B细胞具有在免疫应答的早期阶段产生效应细胞的能力，所述免疫应答针对在该特殊解剖部位中有效清除的颗粒状Ag。
Ag encounter will recruit Ag-specific cells from the pool of mature B lymphocytes in the spleen and activate them to perform effector functions: generation of Ab-forming cells (plasma cells) and presentation of Ag to T cells. We have compared the ability of mature follicular and marginal zone cells to develop into effector B cells. The generation of marginal zone B cells and their localization in the marginal sinus area are T cell and CD40 ligand independent, suggesting that they do not represent a postgerminal center population. Compared with mature recirculating follicular B cells, they express several characteristics of previous antigenic experience, including higher levels of B7.1 (CD80) and B7.2 (CD86) when freshly isolated and following in vitro stimulation. After a brief 6- to 8-h in vitro stimulation with LPS or anti-CD40 Abs, marginal zone B cells become potent APCs. In addition, their ability to proliferate and differentiate into plasma cells in response to low doses of T-independent polyclonal stimuli (LPS) is far greater than that of follicular B cells. These findings indicate a functional heterogeneity within splenic mature B lymphocytes, with marginal zone B cells having the capacity to generate effector cells in early stages of the immune response against particulate Ags scavenged efficiently in this special anatomical site.