GENETIC ANALYSIS OF RECF PATHWAY TO GENETIC RECOMBINATION IN ESCHERICHIA-COLI K12 - ISOLATION AND CHARACTERIZATION OF MUTANTS
GENETIC ANALYSIS OF RECF PATHWAY TO GENETIC RECOMBINATION IN ESCHERICHIA-COLI K12 - ISOLATION AND CHARACTERIZATION OF MUTANTS
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DOI：
10.1016/0022-2836(73)90176-9
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发表时间：
1973-01-01
影响因子：
5.6
通讯作者：
CLARK, AJ
中科院分区：
文献类型：
--
作者：
HORII, ZI;CLARK, AJ
用亚硝基胍诱变大肠杆菌重组菌株recB - recC - sbcB -。在分离的重组缺陷突变体中，1个是sbcB+， 3个是recA ~， 11个是至少4个新rec基因的突变体：recF， recJ， recK和recL。recF143和recf152与ilv共导，但它们位于ilv操作子的两侧，这是F $研究确定的。recF、recL和recK不参与RecBC重组途径，因为携带其中一个基因突变的recB+ recC+ sbcB -菌株是重组熟练的。因此，RecF重组途径可以部分独立地替代RecBC重组途径的假设得到了支持。recF - recB+和recF+ recB -单突变体对紫外辐射敏感，而recF - recB -双突变体比单突变体更敏感。recB - recC - sbcB - recF -菌株的敏感性接近recA -单突变体的敏感性。这被解释为存在部分独立的RecF和RecBC途径来修复紫外线损伤。recJ和突变没有精确映射；因此，它们所赋予的突变特性不能被确定地陈述。
A recombination proficient strain ofEscherichia coli which is recB− recC− sbcB− has been subjected to mutagenesis by nitrosoguanidine. Among the recombination deficient mutants isolated one was sbcB+, three were recA∼ and 11 were mutants in at least four newrec genes: recF, recJ, recK and recL. recF143 and recL152 are cotransducible with ilv but they lie on opposite sides of the ilv operons as determined by F $ ́ studies. recF, recL and recK are not involved in the RecBC pathway of recombination since a recB+ recC+ sbcB− strain carrying a mutation in one of these genes is recombination proficient. Hence the hypothesis that a RecF pathway of recombination can operate as a partially independent substitute for the RecBC pathway of recombination is supported. recF− recB+ and recF+ recB− single mutants are sensitive to uv irradiation while the recF− recB− double mutant is more sensitive than either single mutant. The sensitivity of the recB− recC− sbcB− recF− strain approaches the sensitivity of a recA− single mutant. This is interpreted to mean that there are partially independent RecF and RecBC pathways for the repair of uv damage. recJ and mutations were not mapped precisely; hence the mutant properties they confer can not be stated conclusively.