The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established α, β and δ sub-groups
The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established α, β and δ sub-groups
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DOI：
10.1016/j.plasmid.2006.11.007
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发表时间：
2007-07-01
期刊：
影响因子：
2.6
通讯作者：
Sorensen, Soren J.
中科院分区：
文献类型：
--
作者：
Bahl, Martin Iain;Hansen, Lars Hestbjerg;Sorensen, Soren J.
PKJK5通过外源分离从土壤环境中回收了54,383株对四环素和甲氧苄氨嘧啶具有抗性的菌株。测序和注释显示，主干基因与其他含有Tra1和Tra2区、中央控制模块和复制起始模块的IncP-1质粒具有很高的结构相似性。PKJK5的主干基因与a亚组(3和8个亚组)的同源基因有相当程度的差异，这表明pKJK5可能属于IncP-1质粒的一个新亚组，该亚组也可能包括部分测序的非亚组分类质粒pEMT3。PKJK5中单个骨架基因的GC含量一直低于原型IncP-1的同源基因(3个质粒8751，表明IncP-1的骨架基因同源改良)，平均含量为6.3%。在pKJK5的Tra1和Tra2区域之间有两个分离的辅助元件，分别为2145by(Load 1)和11678 by(Load 2)，它们都由指示转座子介导的插入的反向重复序列和直接侧翼重复序列所包围。Load 1由插入序列ISPa17组成，Load 2是Tn402-衍生物，含有1类整合子IS1326和与含有四环素抗性决定簇的pTB11区域相同的片段和IncP-1αoriV区的一部分。(C)2006 Elsevier Inc.保留所有权利。
The 54,383 by plasmid pKJK5 was recovered from a soil environment by exogenous plasmid isolation and conveys resistance towards tetracycline and trimethoprim. Sequencing and annotation revealed a high level of structural similarity of the backbone genes to other IncP-1 plasmids containing a Tra1 and Tra2 region, a central control module and a replication initiation module. A considerable degree of divergence was associated with the backbone genes of pKJK5 as compared to homologous genes in the a, (3 and 8 subgroups, which indicates that pKJK5 may belong to a novel subgroup of IncP-1 plasmids, which may also accommodate the partially sequenced non-subgroup classified plasmid pEMT3. Individual backbone genes in pKJK5 have a GC-content, which is consistently lower (average 6.3%) than the homologous genes from the archetype IncP-1(3 plasmid 8751 indicating homogenous amelioration of IncP-1 plasmid backbone genes. Two discrete accessory elements of 2145 by (load 1) and 11678 by (load 2) respectively are situated between the Tra1 and Tra2 regions of pKJK5, both bounded by inverted repeats and direct flanking repeats indicative of transposon-mediated insertion. Load 1 consists of an insertion sequence ISPa17 and load 2 is a Tn402-derivative containing a class 1 integron, IS1326 and a fragment identical to a region of plasmid pTB11 harboring -a tetracycline resistance determinant and part of an IncP-1 alpha oriV region. (c) 2006 Elsevier Inc. All rights reserved.