Quantification of Carcinoembryonic Antigen by Capillary Electrophoresis-Chemiluminescence Detection using Internal Standard Method
Quantification of Carcinoembryonic Antigen by Capillary Electrophoresis-Chemiluminescence Detection using Internal Standard Method
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DOI：
10.1080/00032710802568812
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Li, Furong
中科院分区：
文献类型：
--
作者：
Liu, Yanming;Yue, Haiyan;Li, Furong
建立了毛细管电泳联用化学发光法(CE-CL)测定肿瘤标志物癌胚抗原(CEA)的内标法。系统地优化了影响毛细管电泳法分离和化学发光检测的参数。在最佳条件下，以辣根过氧化物酶(HRP)为内标的游离辣根过氧化物酶(HRP)标记的抗体(Ab*)、结合的Ab*-抗原复合体(Ab*-Ag)和HRP在5min内得到很好的分离。该方法已成功地应用于健康人和患者血清中CEA的定量。
Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis-chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal conditions, the free horseradish peroxidase (HRP)-labeled antibody (Ab*), the bound Ab*-antigen complex (Ab*-Ag), and HRP used as internal standard were well separated within 5 min. The proposed method was successfully applied for the quantification of CEA in human sera obtained from both healthy persons and patients.