Reactivity of antibody to ganglioside GD3 against enzootic bovine lymphosarcoma tumor cells.
Reactivity of antibody to ganglioside GD3 against enzootic bovine lymphosarcoma tumor cells.
复制标题
神经节苷脂 GD3 抗体对地方性牛淋巴肉瘤肿瘤细胞的反应性。
DOI：
10.1292/jvms1939.50.463
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Yasuda
中科院分区：
文献类型：
--
作者：
S. Watarai;M. Onuma;T. Yasuda
抗神经节苷脂GD 3抗体在地方性牛淋巴肉瘤（EBL）的肿瘤淋巴结细胞中总是增加，检测其在检测EBL肿瘤细胞中的可能有用性。用EBL肿瘤淋巴结细胞来源的GD 3免疫鸡制备抗GD 3抗体。抗神经节苷脂GD 3抗体与EBL肿瘤细胞中含有N-羟乙酰神经氨酸的神经节苷脂GD 3反应。采用补体依赖性抗体细胞毒性试验检测了抗GD 3抗体与非白血病牛和白血病牛外周血淋巴细胞（PBL）和牛胎儿组织的反应性。神经节苷脂GD 3抗体对EBL牛PBL、胎牛胸腺和肝细胞具有细胞毒活性，但对健康牛PBL无细胞毒活性，无论是否存在牛白血病病毒感染。此外，在间接荧光抗体试验中，丙酮固定的EBL肿瘤细胞对神经节苷脂GD 3的抗体显示阳性反应。
The antibody to ganglioside GD3, which was invariably increased in tumor lymph node cells of enzootic bovine lymphosarcoma (EBL), was examined for its possible usefulness in detecting EBL tumor cells. The anti-GD3 antibody was prepared by immunization of chicken with GD3 from EBL tumor lymph node cells. The antibody to ganglioside GD3 reacted to ganglioside GD3 containing N-glycolylneuraminic acid from EBL tumor cells. The reactivities of anti-GD3 antibody to peripheral blood lymphocytes (PBL) from aleukemic and leukemic cattle and tissues of bovine fetuses were examined by complement-dependent antibody cytotoxicity test. The antibody to ganglioside GD3 had cytotoxic activity to PBL from EBL cattle, fetal bovine thymus and liver cells but not to PBL from healthy cattle irrespective of presence or absence of bovine leukemia virus infection. In addition, acctone-fixed EBL tumor cells showed a positive reaction to the antibody to ganglioside GD3 in an indirect fluorescent antibody test.