Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs
Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs
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DOI：
10.1016/j.ab.2009.01.037
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发表时间：
2009-04-15
影响因子：
2.9
通讯作者：
Ballantyne, Jack
中科院分区：
文献类型：
--
作者：
Hanson, Erin K.;Lubenow, Helge;Ballantyne, Jack
法医案例工作中常规使用的用于鉴定生物液体的血清学方法在时间和样品方面都很昂贵，而且灵敏度和特异性程度也各不相同。最近，使用基于分子遗传学的方法，使用信使RNA（mRNA）分析已被提出，以取代传统的体液鉴定方法。然而，在这些mRNA测定中使用的扩增产物的大小（类似于200-300 nt）可能不适合用于法医案例工作中经常遇到的降解或受损样品。近年来，人们对一类新的小的非编码RNA，microRNA（miRNAs，长度类似于20-25个碱基）的兴趣激增，许多已发表的研究报告称，一些miRNAs以组织特异性方式表达。在这篇文章中，我们提供了第一个全面评估的miRNA表达干燥，法医相关的生物液体，血液，精液，唾液，阴道分泌物，经血，试图确定推定的体液特异性的miRNA。测试的452种人类miRNA中的大多数（与已知miRNA组的67%相似）要么在多种体液中表达，要么根本不表达。尽管如此。我们已经鉴定了一组9种miRNA-miR 451、miR 16、miR 135 b、miR 10 b、miR 658、miR 205、miR 124 a、miR 372和miR 412，它们差异表达的程度使得可以使用少至50 pg的总RNA鉴定法医生物学染色的体液来源。基于miRNA的体液鉴定试验具有高度特异性，因为每种体液的miRNA表达谱与从21种人体组织中获得的miRNA表达谱不同。这项研究的结果提供了一个初步的迹象表明，miRNA谱可能提供一个有前途的替代方法，体液鉴定法医案件。(C)2009 Elsevier Inc. All rights reserved.
The serology-based methods routinely used in forensic casework for the identification of biological fluids are costly in terms of time and sample and have varying degrees of sensitivity and specificity. Recently, the use of a molecular genetics-based approach using messenger RNA (mRNA) profiling has been proposed to supplant conventional methods for body fluid identification. However, the size of the amplification products used in these mRNA assays (similar to 200-300 nt) might not be ideal for use with degraded or compromised samples frequently encountered in forensic casework. Recently, there has been an explosion of interest in a novel class of small noncoding RNAs, microRNAs (miRNAs, similar to 20-25 bases in length), with numerous published studies reporting that some miRNAs are expressed in a tissue-specific manner. In this article, we provide the first comprehensive evaluation of miRNA expression in dried, forensically relevant biological fluids-blood, semen, saliva, vaginal secretions, and menstrual blood-in an attempt to identify putative body fluid-specific miRNAs. Most of the 452 human miRNAs tested (similar to 67% of the known miRNAome) were either expressed in multiple body fluids or not expressed at all. Nevertheless. we have identified a panel of nine miRNAs-miR451, miR16, miR135b, miR10b, miR658, miR205, miR124a, miR372, and miR412-that are differentially expressed to such a degree as to permit the identification of the body fluid origin of forensic biological stains using as little as 50 pg of total RNA. The miRNA-based body fluid identification assays were highly specific because the miRNA expression profile for each body fluid was different from that obtained from 21 human tissues. The results of this study provide an initial indication that miRNA profiling may provide a promising alternative approach to body fluid identification for forensic casework. (C) 2009 Elsevier Inc. All rights reserved.