NORMAL BONE PARTICLES ARE PREFERENTIALLY RESORBED IN THE PRESENCE OF OSTEOCALCIN-DEFICIENT BONE PARTICLES INVIVO
NORMAL BONE PARTICLES ARE PREFERENTIALLY RESORBED IN THE PRESENCE OF OSTEOCALCIN-DEFICIENT BONE PARTICLES INVIVO
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DOI：
10.1007/bf02555901
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发表时间：
1991-07-01
影响因子：
4.2
通讯作者：
LIAN, JB
中科院分区：
文献类型：
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作者：
DEFRANCO, DJ;GLOWACKI, J;LIAN, JB
在破骨细胞性骨吸收的体外模型中，我们先前发现，来自华法林治疗的大鼠的骨钙素缺陷性骨颗粒(BPS)被吸收50%，与正常BPS一样，它们招募的破骨细胞较少，抗酒石酸酸性磷酸酶(TRAP)活性降低。为了确定吸收反应的特异性，我们评估了植入的正常和骨钙素缺乏的BP混合物的命运。正常和华法林治疗的供体大鼠在体内预先标记土霉素，以允许鉴定来自任何来源的BP。将正常的、骨钙素缺乏的和50：50的BPS(标记的或未标记的)混合物植入正常大鼠体内，12天后回收进行酶(TRAP)和非脱钙组织形态计量学分析。掺入土霉素对骨颗粒的吸收无明显影响。恢复的骨钙素缺乏的BP被较少的破骨细胞包围，吸收较少，且含有比正常BP更低的可提取TRAP活性。在具有正常BP和骨钙素缺乏BP的混合BP植入物中，每种类型的骨颗粒引起的组织反应与单独植入时相同。值得注意的是，不同的颗粒引起不同的破骨反应，即使在同一种植体内相邻时，也会被不同程度的吸收。这些数据表明，骨钙素可能作为吸收的底物信号，正常骨钙素不影响邻近骨钙素缺乏的骨钙素缺乏的骨钙素的细胞反应。
In an in vitro model of osteoclastic bone resorption, we previously showed that osteocalcin-deficient bone particles (BPs), derived from warfarin-treated rats, were resorbed 50% as well as normal BPs and that they recruited fewer osteoclastic cells with decreased tartrate-resistant acid phosphatase (TRAP) activity. In order to determine the specificity of the resorption response, we evaluated the fate of implanted mixtures of normal and osteocalcin-deficient BPs. Normal and warfarin-treated donor rats were prelabeled in vivo with oxytetracycline to permit identification of BPs from either source. Normal, osteocalcin-deficient, and 50:50 mixtures of BPs (either labeled or unlabeled) were implanted into normal rats and recovered 12 days later for enzymatic (TRAP) and nondecalcified histomorphometric analyses. The incorporated oxytetracycline had no significant effect on resorption of bone particles. The recovered osteocalcin-deficient BPs were surrounded by fewer osteoclastic cells, were resorbed less, and contained less extractable TRAP activity than normal BPs. In mixed BP implants with normal and osteocalcin-deficient BPs, each type of bone particle elicited the same tissue response as when implanted separately. Remarkably, the different particles evoked dissimilar osteoclastic responses and were resorbed to different extents, even when adjacent within the same implant. These data suggest that osteocalcin may act as a substrate signal for resorption and that osteocalcin in the normal BPs does not influence the cellular response to adjacent osteocalcin-deficient BPs.