A rapid screening color test for glyphosate using dabsyl derivatization
A rapid screening color test for glyphosate using dabsyl derivatization
复制标题
使用达布磺酰衍生化快速筛选草甘膦颜色测试
DOI：
10.1007/s11419-018-00460-z
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发表时间：
2019
影响因子：
2.2
通讯作者：
Ohta Hikoto
中科院分区：
文献类型：
--
作者：
Watanabe Daisuke;Kazui Yuko;Ohta Hikoto
目的草甘膦是世界上最常用的除草剂，也是众多中毒案件的受害者。然而，草甘膦的分析一直很困难，因为它的高极性和两性离子结构，并没有简单的筛选测试草甘膦已被称为。本研究的目的是开发一种快速筛选测试草甘膦。MethodsGlycosylate是可视化的通过dabsyl衍生化引起的颜色变化，和衍生的草甘膦被困在氧化锆涂层的二氧化硅颗粒的表面上的特定的化学相互作用之间的膦酸酯基团的草甘膦和氧化锆表面。草甘膦的存在表示氧化锆表面的颜色变化，这是很容易注意到的肉眼.ResultsThe开发的方法被应用到生物样品（尿液和血清），饮料样品（绿色茶和可乐饮料），和土壤样品。生物样品的检出限为100 μg/mL，饮料样品的检出限为800 μg/mL. Conclusions建立了一种基于颜色试验的草甘膦快速筛查方法。该方法快速，所需样品少于30 μL。灵敏度足以用于大多数中度至重度中毒病例。
PurposeGlyphosate is the most common herbicide in the world, and involved in many poisoning cases. However, the analysis of glyphosate has been difficult because of its high polarity and zwitterionic structure, and no simple screening tests for glyphosate have been known. The purpose of this study was to develop a rapid screening test for glyphosate.MethodsGlyphosate was visualized through a color change induced by dabsyl derivatization, and the derivatized glyphosate was trapped on the surface of zirconia-coated silica particles by specific chemical interaction between a phosphonate group of glyphosate and the zirconia surface. The presence of glyphosate was indicated by the color change of zirconia surface, which was easily noticed by the naked eye.ResultsThe developed method was applied to biological samples (urine and serum), beverage samples (green tea and cola drink), and soil samples. No false positive was observed and the detection limit was 100 μg/mL for the biological samples and 800 μg/mL for the beverage samples.ConclusionsA simple screening method for glyphosate based on a color test was established. The method is rapid and requires less than 30 μL of samples. The sensitivity was sufficient for most cases of moderate to severe poisoning.