Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins.
Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins.
复制标题
14-3-3 蛋白的 epsilon 和 zeta 亚型的克隆和表征。
DOI：
10.1089/dna.1994.13.629
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Klein,DC
中科院分区：
文献类型：
--
作者：
Roseboom,PH;Weller,JL;Babila,T;Aitken,A;Sellers,LA;Moffett,JR;Namboodiri,MA;Klein,DC
Two prominent proteins (30 and 33 kD) in a purified preparation of the sheep pineal gland were studied. Amino acid analysis of tryptic peptides indicated that the 33-kD protein was the ∈ isoform of the 14-3-3 family of proteins, and that the 30-kD protein was the ζ isoform. The sheep pineal gland was found to have six other 14-3-3 isoforms in addition to the ∈ and ζ, suggesting that copurification of the ∈ and ζ forms may reflect the existence of homo- or heterodimers comprised of these isoforms. To characterize 14-3-3 proteins further in the pineal gland, the full sequence of the ∈ isoform and a partial sequence of the ζ isoform were cloned from a rat pineal cDNA library and are reported here. Tissue distribution studies using Western blot analysis revealed that rat pineal and retina have levels of 14-3-3 protein similar to those found in brain, and that relatively low levels occur in other tissues. This investigation also revealed the ∈ isoform was present at high levels in the rat pineal gland early in development and decreased steadily thereafter and that 30-kD isoforms exhibited the inverse developmental pattern.
