Suppression of cartilage matrix gene expression in upper zone chondrocytes of osteoarthritic cartilage
Suppression of cartilage matrix gene expression in upper zone chondrocytes of osteoarthritic cartilage
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DOI：
10.1002/art.1780400323
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发表时间：
1997-03-01
影响因子：
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通讯作者：
Bayliss, MT
中科院分区：
文献类型：
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作者：
Aigner, T;Vornehm, SI;Bayliss, MT
客观的。通过研究 3 种主要软骨成分（II 型胶原蛋白、聚集蛋白聚糖和连接蛋白）的信使 RNA (mRNA) 表达，评估骨关节炎软骨细胞的原位合成代谢活性。方法。在正常和骨关节炎 (OA) 软骨标本的平行切片上进行原位杂交实验和蛋白多糖含量的组织化学分析。结果。与正常样本相比，OA软骨较深区域的大多数软骨细胞显示出mRNA表达增加，特别是II型胶原蛋白的mRNA表达增加，并且在较小程度上增加了聚集蛋白聚糖的mRNA表达，然而，上部区域的软骨细胞对于聚集蛋白聚糖或II型胶原蛋白的mRNA表达基本上呈阴性。连接蛋白mRNA在正常和OA标本中表达较低。结论。这些观察结果表明，抑制上部区域软骨细胞的合成代谢活性有助于在 OA 软骨中观察到的代谢失衡。刺激浅表软骨细胞中的基质合成代谢可能是治疗干预的合适目标。
Objective. To evaluate the anabolic activity of osteoarthritic chondrocytes in situ by investigating the messenger RNA (mRNA) expression of 3 major cartilage components, type II collagen, aggrecan, and link protein.Methods. In situ hybridization experiments and histochemical analysis for proteoglycan content were performed on parallel sections of normal and osteoarthritic (OA) cartilage specimens.Results. Most chondrocytes in the deeper zones of OA cartilage showed an increase in mRNA expression, in particular, of type II collagen and to a lesser extent, aggrecan, compared with normal specimens, However, chondrocytes of the upper zone were largely negative for aggrecan or type II collagen mRNA. The expression of link protein mRNA was low in normal and OA specimens.Conclusion. These observations suggest that suppression of the anabolic activity of chondrocytes in the upper zones contributes to the metabolic imbalance observed in OA cartilage, Stimulation of matrix anabolism in superficial chondrocytes might be a suitable target for therapeutic intervention.