ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR.
ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR.
复制标题
P-糖蛋白和 CFTR 中的 ATP 水解循环和机制。
DOI：
10.1006/scbi.1997.0065
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发表时间：
1997
影响因子：
14.5
通讯作者：
Gadsby,DC
中科院分区：
文献类型：
--
作者：
Senior,AE;Gadsby,DC
P-糖蛋白和CFTR是ABC转运蛋白家族的重要成员。两者都使用ATP，前者驱动药物从细胞中挤出并赋予多药耐药性，后者驱动阴离子通道的打开和关闭。我们比较了目前的工作模型的催化循环和机制的两种蛋白质。在Pgp中，NBD似乎在功能上等同，在CFTR中，它们似乎在功能上不同。在这两种蛋白质中，ATP水解发生在两个NBD中，并且提出两个NBD交替催化。还指出了其他差异和相似之处，为未来的发展提供了想法。
P-glycoprotein and CFTR are prominent members of the ABC Transporter family. Both use ATP, the former to drive extrusion of drugs from cells and confer multidrug-resistance, the latter to drive opening and closing of anion channels. We compare current working models of catalytic cycle and mechanism of the two proteins. In Pgp the NBDs appear functionally equivalent, in CFTR they appear functionally distinct. In both proteins, ATP hydrolysis occurs in both NBDs, and it is proposed that the two NBDs alternate in catalysis. Other differences and similarities are noted, fostering ideas for future developments.