The oral cancer microbiome contains tumor space-specific and clinicopathology-specific bacteria.
The oral cancer microbiome contains tumor space-specific and clinicopathology-specific bacteria.
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DOI：
10.3389/fcimb.2022.942328
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发表时间：
2022
影响因子：
5.7
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中科院分区：
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口腔微生物群和口腔癌之间的串扰尚未得到表征。本研究收集了218例患者的临床病理资料进行分析。对27例患者的唾液标本26份、肿瘤组织表面拭子16份、癌旁正常组织16份、肿瘤外部组织22份、肿瘤内部组织22份、淋巴结10枚进行了16S rRNA基因测序。临床病理资料显示，口腔癌患者口腔内常见致病菌，与糖尿病、肿瘤T分期较晚、有颈部淋巴转移呈正相关。测序数据显示，与邻近正常组织相比，肿瘤外部组织的微生物组具有更大的α多样性，其中梭杆菌、普雷沃特氏菌和卟啉单胞菌所占比例较大，而链球菌所占比例较小。空间特定的微生物组，比较外部肿瘤组织和内部肿瘤组织，显示出多样性的微小差异。外肿瘤组织中梭杆菌、奈瑟氏菌、卟啉单胞菌和别雷沃氏菌含量较高，而内肿瘤组织中普氏杆菌、单胞菌和细小单胞菌较丰富。临床病理特异性微生物组分析发现，阴性和阳性结外转移的差异显著，而不同T期和N期的差异略有不同。T1/T2期和无淋巴转移组Gemella和Bacillales较多，而结外转移阴性组较多见螺旋毛和黄杆菌。综上所述，高通量DNA测序结合临床病理特征有助于我们描述不同疾病发展阶段口腔肿瘤微生物组的特殊模式。
The crosstalk between the oral microbiome and oral cancer has yet to be characterized. This study recruited 218 patients for clinicopathological data analysis. Multiple types of specimens were collected from 27 patients for 16S rRNA gene sequencing, including 26 saliva, 16 swabs from the surface of tumor tissues, 16 adjacent normal tissues, 22 tumor outer tissue, 22 tumor inner tissues, and 10 lymph nodes. Clinicopathological data showed that the pathogenic bacteria could be frequently detected in the oral cavity of oral cancer patients, which was positively related to diabetes, later T stage of the tumor, and the presence of cervical lymphatic metastasis. Sequencing data revealed that compared with adjacent normal tissues, the microbiome of outer tumor tissues had a greater alpha diversity, with a larger proportion of Fusobacterium, Prevotella, and Porphyromonas, while a smaller proportion of Streptococcus. The space-specific microbiome, comparing outer tumor tissues with inner tumor tissues, suggested minor differences in diversity. However, Fusobacterium, Neisseria, Porphyromonas, and Alloprevotella were more abundant in outer tumor tissues, while Prevotella, Selenomonas, and Parvimonas were enriched in inner tumor tissues. Clinicopathology-specific microbiome analysis found that the diversity was markedly different between negative and positive extranodal extensions, whereas the diversity between different T-stages and N-stages was slightly different. Gemella and Bacillales were enriched in T1/T2-stage patients and the non-lymphatic metastasis group, while Spirochaetae and Flavobacteriia were enriched in the extranodal extension negative group. Taken together, high-throughput DNA sequencing in combination with clinicopathological features facilitated us to characterize special patterns of oral tumor microbiome in different disease developmental stages.