Isolation and sequence of a human apolipoprotein CII cDNA clone and its use to isolate and map to human chromosome 19 the gene for apolipoprotein CII.
Isolation and sequence of a human apolipoprotein CII cDNA clone and its use to isolate and map to human chromosome 19 the gene for apolipoprotein CII.
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人载脂蛋白 CII cDNA 克隆的分离和测序及其用于分离载脂蛋白 CII 基因并将其定位到人 19 号染色体的用途。
DOI：
10.1073/pnas.81.10.2945
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发表时间：
1984
影响因子：
11.1
通讯作者：
Breslow,JL
中科院分区：
文献类型：
--
作者：
Jackson,CL;Bruns,GA;Breslow,JL
通过使用对应于载脂蛋白CII氨基酸6-10所有可能密码子的混合寡核苷酸探针筛选成人肝脏cDNA文库，鉴定编码人载脂蛋白CII（apo CII）的cDNA克隆。选择一个具有约等于500个碱基对（bp）插入物的克隆（命名为pCII-711）用于DNA序列分析。该克隆含有与先前报告的载脂蛋白CII氨基酸序列一致的DNA序列，仅存在微小差异。DNA序列指定了79个氨基酸的多肽，而以前报道的是78个氨基酸。pCII-711克隆含有一个36-bp DNA序列，该序列位于指定NH 2-末端苏氨酸的序列上游，当读框时，指定氨基酸序列Leu-Val-Leu-Leu-Val-Leu-Gly-Phe-Glu-Val-Gln-Gly，并且可能是apo CII信号肽的一部分。pCII-711克隆还含有一个144 bp区域，对应于apo CII mRNA的3'非翻译区以及一部分poly（A）尾。克隆pCII-711用于通过限制性内切酶消化从人基因组文库中分离和表征apo CII基因。此外，通过对人-啮齿动物体细胞杂种DNA的Southern印迹分析，克隆pCII-711也被用于将apo CII基因临时定位到人类19号染色体。
cDNA clones encoding human apolipoprotein CII (apo CII) were identified by screening an adult human liver cDNA library with a mixed oligonucleotide probe corresponding to all possible codons for apo CII amino acid 6-10. One clone with an approximately equal to 500-base-pair (bp) insert, designated pCII -711, was selected for DNA sequence analysis. This clone contained a DNA sequence that corresponded with the previously reported amino acid sequence of apo CII with only minor differences. The DNA sequence specified a polypeptide of 79 amino acids, compared to the 78 amino acids previously reported. The pCII -711 clone contains a 36-bp DNA sequence upstream from that specifying the NH2-terminal threonine which, when read in frame, specifies the amino acid sequence Leu-Val-Leu-Leu-Val-Leu-Gly-Phe-Glu-Val-Gln-Gly and may be part of an apo CII signal peptide. The pCII -711 clone also contains a 144-bp region that corresponds to the 3' untranslated region of apo CII mRNA as well as a portion of the poly(A) tail. Clone pCII -711 was used to isolate and characterize by restriction endonuclease digestion the gene for apo CII from a human genomic library. In addition, through Southern blot analysis of DNA from human-rodent somatic cell hybrids, clone pCII -711 also was used to provisionally map the gene for apo CII to human chromosome 19.