Effects of vitamin D3 supplementation and UVb exposure on the growth and plasma concentration of vitamin D3 metabolites in juvenile bearded dragons (Pogona vitticeps)
Effects of vitamin D3 supplementation and UVb exposure on the growth and plasma concentration of vitamin D3 metabolites in juvenile bearded dragons (Pogona vitticeps)
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DOI：
10.1016/j.cbpb.2010.02.008
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Hendriks, W. H.
中科院分区：
文献类型：
--
作者：
Oonincx, D. G. A. B.;Stevens, Y.;Hendriks, W. H.
研究了维生素D-3和紫外线（UVB）暴露对生长期须海龙（Pogona vitticeps）血浆维生素D代谢的影响。共84（40名男性和44名女性）新孵化的胡子龙被分配到6个水平的口服维生素D-3补充（0至400%）或6个UVB暴露时间（2至12小时）。在3月龄和6月龄时，从每只动物中采集血样，并分析25（OH）D-3和1，25（OH）（2）D-3。在3月龄时，与1，25（OH）（2）D-3不同，25（OH）D-3的血浆浓度并不随维生素D-3补充量的增加而增加。在6个月大时，25（OH）D-3和1，25（OH）（2）D-3的血浆浓度随着维生素D-3补充量的增加而增加。暴露于UVB的动物血浆中的25（OH）D-3浓度高出18倍（178.4 +/- 9.0 vs. 9.9 +/- 1.3 nmol/L），1，25（OH）（2）D-3（1.205 +/- 0.100 vs. 0.229 +/- 0.025 nmol/L）比6月龄时补充维生素D-3的动物。这项研究表明，2小时的紫外线照射，使足够的生理浓度的血浆维生素D代谢产物，以保持在不断增长的胡子龙。口服补充维生素D-3不能有效地将25（OH）D-3和1，25（OH）（2）D-3的血浆浓度提高到暴露于UVB的动物中观察到的浓度。(C)2010年爱思唯尔公司All rights reserved.
The effectiveness of dietary vitamin D-3 and UVb exposure on plasma vitamin D metabolites in growing bearded dragons (Pogona vitticeps) was studied. A total of 84 (40 males and 44 females) newly hatched bearded dragons were allocated to six levels of oral vitamin D-3 supplementation (0 to 400%) or six UVb exposure times (2 to 12 h). At 3 and 6 months of age, blood samples were obtained from each animal and analysed for 25(OH)D-3 and 1,25 (OH)(2)D-3. At 3 months of age, plasma concentrations of 25(OH)D-3 did not increase with increasing vitamin D-3 supplementation unlike the 1,25(OH)(2)D-3. At 6 months of age, plasma concentrations of both 25(OH)D-3 and 1,25 (OH)(2)D-3 increased with increasing vitamin D-3 supplementation. Plasma concentrations in UVb-exposed animals were 18 times higher for 25(OH)D-3 (178.4 +/- 9.0 vs. 9.9 +/- 1.3 nmol/L) and 5.3 times higher for 1,25(OH)(2)D-3 (1.205 +/- 0.100 vs. 0.229 +/- 0.025 nmol/L) than in vitamin D-3 supplemented animals at 6 months of age. This study shows that 2 h of UVb exposure enables adequate physiological concentrations of plasma vitamin D metabolites to be maintained in growing bearded dragons. Oral supplementation of vitamin D-3 is ineffective in raising plasma concentrations of 25(OH)D-3 and 1,25(OH)(2)D-3 to concentrations observed in UVb-exposed animals. (C) 2010 Elsevier Inc. All rights reserved.