Transfection of neurotrophin-3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy.
Transfection of neurotrophin-3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy.
复制标题
将神经营养蛋白3转染到神经干细胞中，并使用微泡超声处理，以治疗已取消的肌肉萎缩。
DOI：
10.3892/etm.2017.5439
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发表时间：
2018-01
影响因子：
2.7
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Gong L;Jiang C;Liu L;Wan S;Tan W;Ma S;Jia X;Wang M;Hu A;Shi Y;Zhang Y;Shen Y;Wang F;Chen Y
神经营养素-3（NT-3）具有作为治疗失神经肌肉萎缩患者的治疗剂的潜力。然而，NT-3的内源性分泌低，并且外源性NT-3由于其短的半衰期而缺乏足够的时间来积累。NT-3的转染已被证明对失神经肌肉和运动终板具有有益的作用。神经干细胞（NSC）分化为神经元，形成运动终板神经肌肉连接。先前已经证明，可以使用具有微泡（MB）的超声进行局部和非侵入性转染。在本研究中，苏木精和伊红，乙酰胆碱酯酶和氯化金染色，以及透射电子显微镜，以验证这种治疗策略的效果。结果表明，使用超声与MBs的NT-3到神经干细胞的转染，并随后在体内移植，减轻失神经肌肉的萎缩和减少运动终板退变。因此，这种非侵入性，有效和有针对性的治疗策略可能是一个潜在的治疗失神经肌肉萎缩患者。
Neurotrophin-3 (NT-3) has potential as a therapeutic agent for the treatment of patients with denervated muscle atrophy. However, the endogenous secretion of NT-3 is low and exogenous NT-3 lacks sufficient time to accumulate due to its short half-life. The transfection of NT-3 has been demonstrated to have a beneficial effect on denervated muscle and motor endplates. Neural stem cells (NSCs) differentiate into neurons and form motor endplate nerve-muscle connections. It has been previously demonstrated that local and noninvasive transfection can be performed using ultrasound with microbubbles (MBs). In the current study, hematoxylin and eosin, acetylcholinesterase and gold chloride staining, as well as transmission electron microscopy, were performed to verify the effects of this treatment strategy. The results demonstrated that using ultrasound with MBs for the transfection of NT-3 into NSCs, and their subsequent transplantation in vivo, attenuated the atrophy of denervated muscle and reduced motor endplate degeneration. This noninvasive, efficient and targeted treatment strategy may therefore be a potential treatment for patients with denervated muscle atrophy.
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影响因子：
4.1
作者：
Bost, F;Caron, L;Binétruy, B
通讯作者：
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影响因子：
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