Relation between patterns of intrinsic lateral connectivity, ocular dominance, and cytochrome oxidase-reactive regions in macaque monkey striate cortex.
Relation between patterns of intrinsic lateral connectivity, ocular dominance, and cytochrome oxidase-reactive regions in macaque monkey striate cortex.
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DOI：
10.1093/cercor/6.2.297
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发表时间：
1996-03
期刊：
影响因子：
3.7
通讯作者：
T. Yoshioka;G. Blasdel;J. B. Levitt;J. Lund
中科院分区：
文献类型：
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作者：
T. Yoshioka;G. Blasdel;J. B. Levitt;J. Lund
为了帮助理解猕猴纹状皮层浅层（V1区）中长程、集群横向连接的作用，我们使用基于生物胞素的神经解剖学示踪、CO组织化学和光学成像，检查了内在连接模式与细胞色素氧化酶（CO）斑点、斑点间和眼优势（OD）带的关系。第 3 层中生物胞素的显微注射导致第 1-3 层中标记轴突末端簇的不对称场（平均各向异性为 1.8；最大扩散 -3.7 毫米），标签扩散的长轴方向与斑点行和成像 OD 条纹正交，平行于 V1/V2 边界。这些来自斑点或斑点间注射 (n = 20) 的标记末端斑块 (n = 186) 显示 71%（186 个中的 132 个）斑块与注射部位位于同一隔室； 11%（186 个中的 20 个）到相对的隔室，18%（186 个中的 34 个）到 blob-interblob 隔室的边界，表明连接模式不是严格的 blob 到 blob 或 interblob 到 interblob (p < 0.005; chi(2))。在单个 OD 域 (n = 11) 内的注射中，54% 的标记末端斑块（79 个中的 43 个）落入与注射位点相同的 OD 区域，28%（79 个中的 22 个）落入相反的 OD 区域，18%（79 个中的 14 个）落入边界，显示出对同眼区室的一些连接偏差（p < 0.02；方差分析）。然而，个别注射病例表现出相同隔室连接的程度各不相同（CO 模式为 50-100%，OD 模式为 22-100%）。这些结果表明，虽然相似区室之间的连接占主导地位（例如，斑点到斑点、右眼列到右眼列），但功能不同的区域之间确实发生相互作用。
To help understand the role of long-range, clustered lateral connections in the superficial layers of macaque striate cortex (area V1), we have examined the relationship of the patterns of intrinsic connections to cytochrome oxidase (CO) blobs, interblobs, and ocular dominance (OD) bands, using biocytin based neuroanatomical tracing, CO histochemistry, and optical imaging. Microinjections of biocytin in layer 3 resulted in an asymmetric field (average anisotropy of 1.8; maximum spread--3.7 mm) of labeled axon terminal clusters in layers 1-3, with the longer axis of the label spread oriented orthogonal to the rows of blobs and imaged OD stripes, parallel to the V1/V2 border. These labeled terminal patches (n = 186) from either blob or interblob injections (n = 20) revealed a 71% (132 out of 186) commitment of patches to the same compartment as the injection site; 11% (20 out of 186) to the opposite compartment, and 18% (34 out of 186) to borders of blob-interblob compartments, indicating that the connectivity pattern is not strictly blob to blob, or interblob to interblob (p < 0.005; chi(2)). In injections placed within single OD domains (n = 11), 54% of the resulting labeled terminal patches (43 out of 79) fell into the same OD territories as the injection sites, 28% (22 out of 79) into the opposite OD regions, and 18% (14 out of 79) on borders, showing some connectional bias toward same-eye compartments (p < 0.02; ANOVA). Individual injection cases, however, varied in the degree (50-100% for CO patterns, 22-100% for OD patterns) to which they showed same-compartment connectivity. These results reveal that while connectivity between similar compartments predominates (e.g., blob to blob, right eye column to right eye column), interactions do occur between functionally different regions.