Transcript abundance of the pig stearoyl-CoA desaturase gene has no effect on fatty acid composition in muscle and fat tissues, but its polymorphism within the putative microRNA target site is associated with daily body weight gain and feed conversion ratio
Transcript abundance of the pig stearoyl-CoA desaturase gene has no effect on fatty acid composition in muscle and fat tissues, but its polymorphism within the putative microRNA target site is associated with daily body weight gain and feed conversion ratio
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DOI：
10.2527/jas.2012-5380
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发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Switonski, M.
中科院分区：
文献类型：
--
作者：
Bartz, M.;Szydlowski, M.;Switonski, M.
猪肌内脂肪中的脂肪酸组成影响肉的食用价值和工艺特性。硬脂酰辅酶A去饱和酶（SCD）基因是脂肪酸组成的强有力的位置和功能候选基因。我们对 4 个品种（杜洛克、皮特兰、波兰长白和波兰大白）的序列分析揭示了 5' 侧翼序列中的一个新 SNP，以及 3' 非翻译区 (UTR) 中的 9 个新 SNP 和 2 个新插入缺失。皮下脂肪中的 SCD 转录水平显着高于肌肉组织 (n = 83; P < 0.001)，并且品种间比较显示波兰长白猪脂肪组织中的 SCD 转录水平更高 (P < 0.01)。我们发现任何组织中 SCD 转录本的丰度与脂肪酸组成之间没有关联。我们对 4 个 SNP（c.-353C > T、c.-233T > C、c.*164A > G 和 c.*928G > C）、1 个插入缺失 (c.*2574_2576delGTC) 以及波兰大白鲑 (n = 185) 和合成品系 990 (n = 243) 的生产性状进行了关联分析。观察到最明显的关联是 c.*928G > C 多态性，该多态性发生在 2 个 microRNA（ssc-miR-185 和 ssc-miR-491）的预测靶位点内。在990系中，这种多态性与日体重增加（一般模型下P<0.04）和饲料转化率（P<0.0004）显着相关，但与脂肪性状无关。在波兰大白品种中也观察到了同样的趋势，但并不显着。当两个品种一起分析时，这些关联再次非常显着（每日体重增加 P < 0.003；饲料转化率 P < 0.0001）。我们得出结论，c.*928G > C 是猪这两种性状的有希望的标记。 (C) 2013 年美国动物科学学会。版权所有。
Fatty acid composition in porcine intramuscular fat affects the dietetic value and technological properties of meat. The stearoyl-CoA desaturase (SCD) gene is a strong positional and functional candidate for fatty acid composition. Our sequence analysis in 4 breeds (Duroc, Pietrain, Polish Landrace, and Polish Large White) revealed a novel SNP in the 5'-flanking sequence and 9 novel SNP and 2 novel indels in the 3' untranslated region (UTR). Transcript level of the SCD in subcutaneous fat was significantly greater than in muscle tissue (n = 83; P < 0.001) and the interbreed comparison revealed a greater transcript level in the fat tissue of Polish Landrace (P < 0.01). We found no association between the abundance of the SCD transcript and fatty acid composition in any of the tissues. We performed an association analysis between 4 SNP (c.-353C > T, c.-233T > C, c.*164A > G, and c.*928G > C), 1 indel (c.*2574_2576delGTC), and production traits in Polish Large White (n = 185) and synthetic line 990 (n = 243). The most pronounced associations were observed for the c.*928G > C polymorphism, which occurs within a predicted target site for 2 microRNA (ssc-miR-185 and ssc-miR-491). In line 990, this polymorphism was significantly associated with daily BW gain (P < 0.04 under the general model) and feed conversion ratio (P < 0.0004) but not with fatness traits. The same tendency, but not significant, was observed in the Polish Large White breed. When both breeds were analyzed together, these associations were again highly significant (daily BW gain P < 0.003; feed conversion ratio P < 0.0001). We conclude that c.*928G > C is a promising marker for both porcine traits. (C) 2013 American Society of Animal Science. All rights reserved.