motA1552, a mutation of Dictyostelium discoideum having pleiotropic effects on motility and discoidin I regulation.
motA1552, a mutation of Dictyostelium discoideum having pleiotropic effects on motility and discoidin I regulation.
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motA1552，盘基网柄菌的突变体，对运动和盘基蛋白 I 调节具有多效性作用。
DOI：
10.1093/genetics/118.3.425
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Clarke,M
中科院分区：
文献类型：
--
作者：
Kayman,SC;Birchman,R;Clarke,M
盘基网柄菌突变体 MC2 表现出温度敏感性生长、温度敏感性运动和盘基蛋白 I 合成的温度诱导。在此报道的遗传实验中，MC2 的这三种表型并未分开。因此，它们被分配至突变motA1552，通过分离分析和有丝分裂重组二倍体分析将其定位至连锁群II。在一种 motA1552 菌株中，运动性丧失先于盘状蛋白 I 积累 3 小时，表明盘状蛋白 I 不参与运动缺陷的产生。 motA1552 表型的表达在携带突变的菌株之间以及特定菌株的不同克隆之间存在差异。 MC2 及其衍生物在连锁群 II 上显示出whA 和acrA 之间重组水平升高，并在acrA 基因座处产生高度不稳定的突变。发现在菌株 AX3 的无菌生长过程中大量盘状蛋白 I 的积累取决于第二个连锁组 II 突变 daxA1551 的存在。这种突变已经存在于经诱变分离 motA1552 的菌株中，使 motA1552 作用的解释变得复杂化。
The Dictyostelium discoideum mutant MC2 exhibits temperature-sensitive growth, temperature-sensitive motility, and temperature induction of discoidin I synthesis. These three phenotypes of MC2 were not separated in the genetic experiments reported here. They were therefore assigned to the mutation motA1552, which was mapped to linkage group II by segregation analysis and by analysis of mitotic recombinant diploids. In one motA1552 strain, loss of motility preceded accumulation of discoidin I by 3 hr, indicating that discoidin I is not involved in generation of the motility defect. Expression of motA1552 phenotypes varied both among strains carrying the mutation, and among different clones of a particular strain. MC2 and its derivatives displayed elevated levels of recombination between whiA and acrA on linkage group II, and yielded highly unstable mutations at the acrA locus. Accumulation of large amounts of discoidin I during axenic growth of strain AX3 was found to depend on the presence of a second linkage group II mutation, daxA1551. This mutation was already present in the strain mutagenized to isolate motA1552, complicating explication of motA1552 action.