One-and-a-half wavelength approach
One-and-a-half wavelength approach
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DOI：
10.1107/s0907444902016645
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Dauter, Z
中科院分区：
文献类型：
--
作者：
Dauter, Z
在许多情况下，单波长异常衍射（SAD）定相导致成功的结构解，但是不可能预先预测具有一定量的异常信号的单波长数据是否足够。因此，根据MAD协议继续在不同波长处收集数据，但同时尝试通过SAD方法对第一数据集进行定相是更安全的。如果成功，则可以放弃进一步的数据收集。这种“1.5波长”方法可以节省大量的时间和精力，并减少晶体辐射损伤的影响。SAD定相的原理使用Argand平面中的矢量图来说明。
In many cases single-wavelength anomalous diffraction (SAD) phasing leads to a successful structure solution, but it is impossible to predict beforehand if single-wavelength data with a certain amount of anomalous signal resulting will be sufficient. It is therefore safer to continue collecting data at different wavelengths according to a MAD protocol, but to simultaneously attempt to phase the first data set by the SAD method. If this is successful, then further data collection can be abandoned. This '1.5-wavelength' approach may save a substantial amount of time and effort and diminishes the effects of crystal radiation damage. The principles of SAD phasing are illustrated using vector diagrams in the Argand plane.