Microsatellite instability(MSI) in non-small cell lung cancer(NSCLC) is highly associated with transforming growth factor-beta type II receptor(TGF-beta RII) frameshift mutation.
Microsatellite instability(MSI) in non-small cell lung cancer(NSCLC) is highly associated with transforming growth factor-beta type II receptor(TGF-beta RII) frameshift mutation.
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发表时间：
2000-05
影响因子：
2
通讯作者：
W. Kim;C. Park;S. K. Hong;B. Park;H. Kim;K. Park
中科院分区：
文献类型：
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作者：
W. Kim;C. Park;S. K. Hong;B. Park;H. Kim;K. Park
与微卫星不稳定性（MSI）相关的TGF-β II型受体（TGF-β RII）突变是10 bp聚腺苷酸序列内的特征性移码突变。据报道，这些移码突变在结直肠癌和胃癌MSI中很常见，但在非小细胞肺癌（NSCLC）中很少报道。材料与方法在这项研究中，我们分析了MSI和TGF-β RII移码突变在7个NSCLC细胞系和21个手术切除的NSCLC组织。使用BAT-25、BAT-26和BAT-40的引物组进行NSCLC中MSI的测定。为了检查MSI样品中TGF-β RII的移码突变的存在，对TGF-β RII多聚A片段进行测序。结果7株NSCLC细胞系中有5株发生MSI，21例NSCLC组织中有3例发生MSI。8例MSI中有6例（75%）显示TGF-β RII poly-A区发生移码突变。结论非小细胞肺癌中MSI与TGF-β RII移码突变密切相关，进一步支持TGF-β RII在非小细胞肺癌发生中起重要作用的假说。
BACKGROUND TGF-beta type II receptor (TGF-beta RII) mutations associated with microsatellite instability(MSI) are characteristically frameshift mutations within a 10 bp poly-A tract. These frameshift mutations have been reported to be common in colorectal and gastric cancers with MSI, though, rarely reported in non-small cell lung cancer (NSCLC). MATERIALS AND METHOD In this study, we analysed MSI and TGF-beta RII frameshift mutations in 7 NSCLC cell lines and 21 surgically resected NSCLC tissues. Determination of MSI in NSCLC was performed using primer sets for BAT-25, BAT-26 and BAT-40. In order to examine the presence of the frameshift mutations of TGF-beta RII in samples with MSI, sequencing for TGF-beta RII poly-A tract was performed. RESULTS MSI was observed in 5 out of 7 NSCLC cell lines and 3 out of 21 NSCLC tissues. Six out of 8 samples with MSI(75%) showed frameshift mutations in TGF-beta RII poly-A tract. CONCLUSION These results suggest that MSI is highly associated with TGF-beta RII frameshift mutations in NSCLC and further support the hypothesis that TGF-beta RII plays an important role in NSCLC carcinogenesis.