Long noncoding RNAs interact with mRNAs: A new perspective on the mechanism of premature brain injury
Long noncoding RNAs interact with mRNAs: A new perspective on the mechanism of premature brain injury
复制标题
长非编码RNA与mRNA相互作用：过早脑损伤机制的新视角
DOI：
10.1016/j.neulet.2019.05.028
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发表时间：
2019-08-10
影响因子：
2.5
通讯作者：
Liu, Li
中科院分区：
文献类型：
--
作者：
Chen, Rujuan;Piao, Xianhua;Liu, Li
炎症引起的过早脑损伤的分子机制尚不完全清楚。据报道，长非编码 RNA (lncRNA) 在包括脑损伤在内的神经系统疾病中发挥着至关重要的作用。然而，人们对 lncRNA 在早产儿大脑中的调节功能知之甚少。本研究调查了早产儿大脑中差异表达的 lncRNA 和 mRNA 及其相互作用。脂多糖（LPS）被用来诱导早产啮齿动物模型的炎症。通过苏木精和伊红（HE）染色和CD68免疫染色观察脑组织学。 Arraystar 微阵列设计用于分析 4 个 LPS 诱导的早产大脑（L 组）、4 个足月对照大脑（C 组）和 3 个未由 LPS 诱导的早产大脑（P 组）中差异表达的 lncRNA 和 mRNA。应用生物信息学分析揭示lncRNA和mRNA的功能和共表达关系。选择 3 个 lncRNA 和 2 个 mRNA 进行应用定量实时聚合酶链反应 (qRT-PCR) 的验证。本研究证明了早产儿大脑在炎症损伤时lncRNA和mRNA谱发生失调，从而从lncRNA和mRNA共表达网络的新角度揭示了早产儿大脑发育的新机制，并为早产儿脑损伤的治疗提供了重要见解。
The molecular mechanism of premature brain injury induced by inflammation is not fully understood. Long noncoding RNAs (lncRNAs) have been reported to play crucial roles in neurological disorders including brain injury. However, little is known about the regulatory function of lncRNAs in the premature brain. This study investigates differentially expressed lncRNAs and mRNAs as well as their interactions in the premature brain. Lipopolysaccharides (LPS) were used to induce inflammation in premature rodent models. Brain histology was observed via hematoxylin and eosin (HE) staining and CD68 immunostaining. Arraystar microarry was designed for the profiling of differentially expressed lncRNAs and mRNAs in 4 LPS induced premature brains (L group), 4 full-term control brains (C group) and 3 premature brains were not induced by LPS (P group). Bioinformatic analysis was applied to reveal the functions and co-expression relationship of lncRNAs and mRNAs. Three lncRNAs and 2 mRNAs were selected for validation applying quantitative real time polymerase chain reaction (qRT-PCR). This study demonstrates dysregulated lncRNA and mRNA profiles in the premature brains upon inflammatory insult, thus revealing a novel mechanism of premature brain development from a new perspective of the lncRNAs and mRNA coexpression network and providing important insights into the therapy of premature brain injury.