Sweat gland organoids contribute to cutaneous wound healing and sweat gland regeneration
Sweat gland organoids contribute to cutaneous wound healing and sweat gland regeneration
复制标题
汗腺类器官有助于皮肤伤口愈合和汗腺再生
DOI：
10.1038/s41419-019-1485-5
复制
发表时间：
2019-03-11
影响因子：
9
通讯作者：
Wang, Yunfang
中科院分区：
文献类型：
--
作者：
Diao, Jinmei;Liu, Juan;Wang, Yunfang
在哺乳动物中，汗腺具有重要的体温调节功能。像其他皮肤成分一样，它们起源于表皮祖细胞。然而，它们对损伤的反应具有较低的再生潜力。我们已经建立了一种使用3D类器官培养物的汗腺培养和扩增方法。将成年小鼠足垫真皮层来源于汗腺的上皮细胞包埋在Matrigel中形成汗腺类器官。这些类器官保持了显著的干细胞特征，并表现出产生汗腺细胞（SGCs）或表皮细胞的分化能力。此外，在为皮肤表皮细胞定制的培养基中，双能SGO衍生的细胞可以在气-液界面培养中被诱导为复层表皮结构。包埋在为汗腺量身定制的Matrigel中的SGCs形成了上皮类器官，其表达汗腺特异性标记物，例如细胞角蛋白（CK）18和CK 19、水通道蛋白（AQP）5和α ATP。更重要的是，将其分别移植到有汗腺损伤的小鼠背部创面和爪垫中，具有表皮和汗腺再生的潜力。综上所述，我们建立并优化了有效产生小鼠链球菌的培养条件。这些细胞是恢复受损的汗腺组织以及改善皮肤再生的候选细胞。
Sweat glands perform a vital thermoregulatory function in mammals. Like other skin components, they originate from epidermal progenitors. However, they have low regenerative potential in response to injury. We have established a sweat gland culture and expansion method using 3D organoids cultures. The epithelial cells derived from sweat glands in dermis of adult mouse paw pads were embedded into Matrigel and formed sweat gland organoids (SGOs). These organoids maintained remarkable stem cell features and demonstrated differentiation capacity to give rise to either sweat gland cells (SGCs) or epidermal cells. Moreover, the bipotent SGO-derived cells could be induced into stratified epidermis structures at the air-liquid interface culture in a medium tailored for skin epidermal cells in vitro. The SGCs embedded in Matrigel tailored for sweat glands formed epithelial organoids, which expressed sweat-gland-specific markers, such as cytokeratin (CK) 18 and CK19, aquaporin (AQP) 5 and alpha ATP. More importantly, they had potential of regeneration of epidermis and sweat gland when they were transplanted into the mouse back wound and claw pad with sweat gland injury, respectively. In summary, we established and optimized culture conditions for effective generation of mouse SGOs. These cells are candidates to restore impaired sweat gland tissue as well as to improve cutaneous skin regeneration.