Survey of genome size in 28 hydrothermal vent species covering 10 families
Survey of genome size in 28 hydrothermal vent species covering 10 families
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DOI：
10.1139/g09-027
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发表时间：
2009-06-01
期刊：
影响因子：
3.1
通讯作者：
Higuet, Dominique
中科院分区：
文献类型：
--
作者：
Bonnivard, Eric;Catrice, Olivier;Higuet, Dominique
了解基因组大小是开发许多基因组资源的有用且必要的先决条件。为了更好地了解物种间 DNA 增加和减少的起源和影响，从广泛的分类学基础以及特定的生态系统中收集数据非常重要。海洋热喷口是研究非常不稳定环境中基因组大小的有趣模型。在这里，我们提供了通过流式细胞术估算的 28 个热液喷口生活物种的数据，这些物种是来自不同热液喷口的最具代表性的物种。我们还报告了密切相关的沿海十足目动物的基因组大小。将单倍体 C 值与先前报道的相应目或下目物种的 C 值进行比较。这是对喷口生物 2C 值的首次广泛调查。与预期相反，它表明某些热液喷口物种具有特别大的基因组。深蹲龙虾 Munidopsisecta 拥有迄今为止报道的任何异壁动物中最大的基因组：2C = 31.1 pg = 30.4 x 10(9) bp。在短尾目、叶齿目和温带目等几个类群中，喷口物种的基因组明显位于各类群已发表值的高端。我们还描述了 Brachyura（沿海蟹 Xantho pilipes 和 Necora puber）迄今为止记录的最高 DNA 含量。最后，对不同群体的基因组大小变异的分析揭示了孔雀虾 Mirocaris fortunata 中意想不到的种内变异，这种变异不能简单地归因于倍性变化。
Knowledge of genome size is a useful and necessary prerequisite for the development of many genomic resources. To better understand the origins and effects of DNA gains and losses among species, it is important to collect data from a broad taxonomic base, but also from particular ecosystems. Oceanic thermal vents are an interesting model to investigate genome size in very unstable environments. Here we provide data estimated by flow cytometry for 28 vent-living species among the most representative from different hydrothermal vents. We also report the genome size of closely related coastal decapods. Haploid C-values were compared with those previously reported for species from corresponding orders or infraorders. This is the first broad survey of 2C values in vent organisms. Contrary to expectations, it shows that certain hydrothermal vent species have particularly large genomes. The vent squat lobster Munidopsis recta has the largest genome yet reported for any anomuran: 2C = 31.1 pg = 30.4 x 10(9) bp. In several groups, such as Brachyura, Phyllodocida, and Veneroida, vent species have genomes that clearly rank at the high end of published values for each group. We also describe the highest DNA content yet recorded for the Brachyura (coastal crabs Xantho pilipes and Necora puber). Finally, analysis of genome size variation across populations revealed unexpected intraspecific variation in the vent shrimp Mirocaris fortunata that could not be attributed simply to ploidy changes.