High-Yield Production of Biohybrid Microalgae for On-Demand Cargo Delivery
High-Yield Production of Biohybrid Microalgae for On-Demand Cargo Delivery
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DOI：
10.1002/advs.202001256
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发表时间：
2020-07-02
期刊：
影响因子：
15.1
通讯作者：
Sitti, Metin
中科院分区：
文献类型：
--
作者：
Akolpoglu, Mukrime Birgul;Dogan, Nihal Olcay;Sitti, Metin
生物混合微泳者利用游动微生物的游动和导航来靶向和递送货物分子，在广泛的生物医学应用中。医用生物混合微型游泳器的制造产量低，这将大大限制其潜在的应用。本文报道了一种以莱茵衣藻（一种单细胞绿色菌）为生物模型微生物，以聚合物-纳米颗粒为合成组分，沿着一层薄而柔软的均匀包被层的生物杂化设计策略，制备效率接近90%.天然生物聚合物壳聚糖用作粘合剂，以有效地用纳米颗粒涂覆微藻的细胞壁。柔软的表面涂层不会损害微藻的生存力和趋光能力，并允许进一步工程化以容纳生物医学货物分子。此外，通过将嵌入薄涂层中的纳米颗粒与化疗阿霉素通过光可裂解的接头缀合，将药物按需递送至肿瘤细胞被报道为概念验证的生物医学证明。高通量策略可以为下一代微型机器人群铺平道路，用于未来的医疗主动货物交付任务。
Biohybrid microswimmers exploit the swimming and navigation of a motile microorganism to target and deliver cargo molecules in a wide range of biomedical applications. Medical biohybrid microswimmers suffer from low manufacturing yields, which would significantly limit their potential applications. In the present study, a biohybrid design strategy is reported, where a thin and soft uniform coating layer is noncovalently assembled around a motile microorganism.Chlamydomonas reinhardtii(a single-cell green alga) is used in the design as a biological model microorganism along with polymer-nanoparticle matrix as the synthetic component, reaching a manufacturing efficiency of approximate to 90%. Natural biopolymer chitosan is used as a binder to efficiently coat the cell wall of the microalgae with nanoparticles. The soft surface coating does not impair the viability and phototactic ability of the microalgae, and allows further engineering to accommodate biomedical cargo molecules. Furthermore, by conjugating the nanoparticles embedded in the thin coating with chemotherapeutic doxorubicin by a photocleavable linker, on-demand delivery of drugs to tumor cells is reported as a proof-of-concept biomedical demonstration. The high-throughput strategy can pave the way for the next-generation generation microrobotic swarms for future medical active cargo delivery tasks.