The Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-type element of the hobo, Ac, and Tam3 (hAT) element family.
The Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-type element of the hobo, Ac, and Tam3 (hAT) element family.
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来自家蝇 Musca Domestica 的 Hermes 转座元件是 hobo、Ac 和 Tam3 (hAT) 元件家族的短反向重复型元件。
DOI：
10.1017/s0016672300032699
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
O'Brochta,DA
中科院分区：
文献类型：
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作者：
Warren,WD;Atkinson,PW;O'Brochta,DA
家蝇的基因组包含一个活跃的转座因子系统，称为爱马仕。利用PCR和反向PCR技术，我们扩增并测序了几个爱马仕元件的重叠片段，从这些数据中，我们构建了一个2749 bp的共有爱马仕DNA序列。爱马仕末端由17 bp的不完全反向重复序列组成，其与果蝇的流浪汉元件的反向末端重复序列几乎相同。全长爱马仕元件含有能够编码612个氨基酸的蛋白质的单个长ORF，其与流浪汉转座酶的氨基酸序列具有55%的同一性。比较爱马仕和流浪汉元件的末端与玉米的Ac元件、金鱼草的Tam3元件以及其他几种植物和昆虫元件的末端，揭示了一个保守的末端序列基序。因此，爱马仕显然是流浪汉、Ac和Tam3（hAT）转座因子家族的成员，该家族的其他成员包括来自拟南芥的Tag1因子和来自玉米的Bg因子。这类转座因子的进化和爱马仕作为遗传工具在M.讨论了昆虫和相关物种。
The genome of the house fly, Musca domestica, contains an active transposable element system, called Hermes. Using PCR and inverse PCR we amplified and sequenced overlapping segments of several Hermes elements and from these data we have constructed a 2749 bp consensus Hermes DNA sequence. Hermes termini are composed of 17 bp imperfect inverted repeats that are almost identical to the inverted terminal repeats of the hobo element of Drosophila melanogaster. Full length Hermes elements contain a single long ORF capable of encoding a protein of 612 amino acids which is 55% identical to the amino acid sequence of the hobo transposase. Comparison of the ends of the Hermes and hobo elements to those of the Ac element of Zea mays, and the Tam3 element of Antirrhinum majus, as well as several other plant and insect elements, revealed a conserved terminal sequence motif. Thus Hermes is clearly a member of the hobo, Ac and Tam3 (hAT) transposable element family, other members of which include the Tagl element from Arabidopsis thaliana and the Bg element from Zea mays. The evolution of this class of transposable elements and the potential utility of Hermes as a genetic tool in M. domestica and related species are discussed.