Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion
Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion
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DOI：
10.1038/s41598-020-62091-y
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发表时间：
2020-03-24
影响因子：
4.6
通讯作者：
Ziyyat, Ahmed
中科院分区：
文献类型：
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作者：
Barbaux, Sandrine;Ialy-Radio, Come;Ziyyat, Ahmed
已知有三个基因对配子黏附/融合是必需的(CD9、Izumo1和Juno)。在这里，我们证实Spaca6缺失的雄性是不育的，并表明他们的精子聚集在卵黄周空间，但无法与卵母细胞融合。与IZUMO1一样，SPACA6在人类精子中表达，顶体反应后残留在赤道段，由于抗SPACA6抗体的抑制，SPACA6参与了人类受精。尽管Spaca6和Izumo1基因敲除导致的表型相似，但这些都不是多余的，IZUMO1的基本重新定位不受SPACA6缺失的影响。我们提出了一个模型，在这个模型中，IZUMO1和SPACA6将是配子融合所必需的分子复合体的一部分，并且它们的伴随存在将需要招募另一个重要的分子参与者，如FusoGen，以进行融合。
Three genes are known to be essential for gamete adhesion/fusion (Cd9, Izumo1 and Juno). Here, we confirmed that Spaca6 null males are infertile and showed that their sperm accumulate in the perivitelline space but are unable to fuse with oocyte. Like IZUMO1, SPACA6 which is expressed by human sperm, is remained on the equatorial segment after acrosomal reaction and is involved in human fertilization since an anti-SPACA6 antibody inhibited it. Despite the similarity of the phenotypes caused by Spaca6 and Izumo1 knockouts, these are not redundant and the essential relocation of IZUMO1 is not affected by the lack of SPACA6. We propose a model in which IZUMO1 and SPACA6 would be part of a molecular complex necessary for gamete fusion and that their concomitant presence would be required for the recruitment of another essential molecular actor, such as a fusogen, for the fusion to take place.