Hyperbaric oxygenation pretreatment induces catalase and reduces infarct size in ischemic rat myocardium
Hyperbaric oxygenation pretreatment induces catalase and reduces infarct size in ischemic rat myocardium
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DOI：
10.1007/s004240100571
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发表时间：
2001-07-01
影响因子：
4.5
通讯作者：
Kim, MS
中科院分区：
文献类型：
--
作者：
Kim, CH;Choi, H;Kim, MS
缺血再灌注损伤是心脏病、体外循环手术和心脏移植中发生的主要并发症。再灌注阶段产生的活性氧压倒了抗氧化酶的清除能力，导致心肌氧化损伤。我们检查了高压氧（HBO）预处理是否会诱导抗氧化酶并保护心脏免受随后的缺血再灌注损伤。每天将大鼠间歇性暴露于 3 ATA（其中 ATA 为绝对大气）的 100% O-2 中 1 小时，然后在室内空气中恢复 24 小时后处死。离体心脏进行 40 分钟的缺血和 90 分钟的再灌注。研究发现 HBO 预处理可以调节心脏并增强过氧化氢酶的酶活性和基因表达，从而显着减少再灌注后的梗塞面积。过氧化氢酶抑制剂3-氨基-1,2,4-三唑完全消除了HBO预处理的梗死限制作用，这表明HBO诱导的对缺血再灌注损伤的耐受性是由于过氧化氢酶的诱导。我们的结果表明 HBO 预处理可能被开发为心脏再灌注损伤的新预防措施。
Ischemia-reperfusion injury is a major complication occurring in heart stroke, cardiopulmonary bypass surgeries, and heart transplantation. Reactive oxygen species generated during the reperfusion phase overwhelm the scavenging capacities of antioxidant enzymes, and result in oxidative damage to the myocardium. We examined whether hyperbaric oxygenation (HBO) pretreatment induces antioxidant enzymes and protects the heart from subsequent ischemia-reperfusion injury. Rats were intermittently exposed to 100% O-2 at 3 ATA (where ATA is absolute atmosphere) for 1 h daily and then sacrificed after 24 h of recovery in room air. Isolated hearts were subjected to 40 min of ischemia and 90 min of reperfusion. HBO pretreatment was found to condition the heart and enhance enzymatic activity and gene expression of catalase, thereby significantly reducing infarct size after reperfusion. A catalase inhibitor, 3 -amino-1,2,4-triazole, completely abolished the infarct-limiting effect of HBO pretreatment, which suggests that HBO-induced tolerance against ischemia-reperfusion injury is due to catalase induction. Our results imply that HBO preconditioning may be developed as a new preventive measure for reperfusion injury in the heart.