Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes
Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes
复制标题
紧密连接的破坏导致 2 型糖尿病小鼠模型唾液腺分泌不足
DOI：
10.1111/joa.13203
复制
发表时间：
2020-05-06
影响因子：
2.4
通讯作者：
Xiang, Ruo-Lan
中科院分区：
文献类型：
--
作者：
Huang, Yan;Mao, Qian-Ying;Xiang, Ruo-Lan
Tight junction (TJ) plays an important role in regulating paracellular fluid transport in salivary glands; however, little is known about the involvement of TJs in diabetes salivary glands. This study aimed to investigate the alterations of TJs and their possible contribution in diabetes-induced hyposalivation. Here, we observed that the morphologies of submandibular glands (SMGs) were impaired, characterized by enlarged acini accumulation with giant secretory granules, which were significantly reduced in atrophic ducts in SMGs of db/db mice, a spontaneous model of type-2 diabetes. However, the secretory granules were increased and scattered in the acini of diabetes parotid glands (PGs). Other ultrastructural damages including swollen mitochondria, expansive endoplasmic reticulum, and autophagosomes were observed in the diabetes group. The levels of TJ proteins including claudin-1 (Cldn1) and claudin-3 (Cldn3) were increased, whereas those of claudin-4 (Cldn4), occludin (Ocln), and zonula occludens-1 (ZO-1) were decreased in SMGs of db/db mice. Higher Cldn1 and Cldn3 and lower claudin-10 (Cldn10) and Ocln levels were observed in PGs of diabetes mice. Taken together, the structures of SMGs and PGs were impaired in diabetes mice, and the disruption of TJ integrity in both SMGs and PGs may contribute to diabetes-induced hyposalivation.
