Protocol for broccoli microspore culture
Protocol for broccoli microspore culture
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DOI：
10.1007/978-94-017-1293-4_30
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
C. J.;Silva Dias
中科院分区：
文献类型：
--
作者：
C. J.;Silva Dias
Broccoli (Brassica oleraceavar.italica) is a vegetable cole crop of considerable interest to breeders and seed companies since its area of production has increased in recent years mainly due to improved nutritional qualities, e.g. anticancer properties. Breeding companies strive more and more to bring F1hybrid seeds onto the market. F1cultivars ensure high uniformity, offer high yields due to heterosis, allow rapid production and selection of desired genotypes and provide protection of plant breeder’s rights and the markets of seed companies. The production of hybrid cultivars requires homozygous parental lines. Inbred lines in broccoli can be produced by recurrent selfing, a procedure that takes time (6 to 7 generations of selfing) and is labour intensive due to sporophitic self-incompatibility. An alternative way to obtain pure inbred lines in one generation is the production of doubled haploid (DH) lines by microspore culture. This protocol can also be applied to other cole crops such as head and tronchuda cabbages using small adaptations.
