Functional characterization of visual opsin repertoire in Medaka (Oryzias latipes)
Functional characterization of visual opsin repertoire in Medaka (Oryzias latipes)
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DOI：
10.1016/j.gene.2005.12.005
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发表时间：
2006-04-26
期刊：
影响因子：
3.5
通讯作者：
Kawamura, S
中科院分区：
文献类型：
--
作者：
Matsumoto, Y;Fukamach, S;Kawamura, S
鱼类的视色素种类繁多，是由于光环境下的水环境变化很大。一套完整的视蛋白基因已经被分离出来，并被表征为仅在斑马鱼视网膜上的吸收光谱和表达。Medaka （Orjlzias latipes）是一种在系统发育上与斑马鱼相距较远的鱼类，是一种重要的脊椎动物分子和发育遗传学模型系统。我们之前分离了一个水母杆视蛋白基因（R-HI）。本研究通过噬菌体和细菌人工染色体（BAC）文库的基因组筛选，分离了medaka的所有视锥蛋白基因。medaka基因组包含2个红色的LWS-A和LWS-B， 3个绿色的RH2-A、RH2-B和RH2-C， 2个蓝色的SWS2-A和SWS2-B亚型视蛋白基因，以及一个单拷贝的紫外视蛋白基因SWS1。以前，在一项基于dna的研究中，只有一个基因被认为存在于每种视蛋白类型。这些亚型视蛋白基因紧密相连，一定是局部基因复制的产物，而不是全基因组复制的产物。含11顺式视网膜的光色素的峰值吸收光谱（λ (max)）在3种绿色视蛋白之间差异较大，分别为RH2-A 452nm、RH2-B 516nm和RH2-C 492nm；在2种蓝色视蛋白之间差异较大，分别为SWS2-A 439nm和SWS2-B 405 nm。斑马鱼也有多种视蛋白亚型，但系统发育分析显示，medaka和斑马鱼独立获得了视蛋白亚型。本研究分离的lambda和BAC DNA克隆可用于研究鱼类视蛋白基因的调控机制和进化多样性。(c) 2005 Elsevier B.V.版权所有
A variety of visual pigment repertoires present in fish species is believed due to the great variation under the water of light environment. A complete set of visual opsin genes has been isolated and characterized for absorption spectra and expression in the retina only in zebrafish. Medaka (Orjlzias latipes) is a fish species phylogenetically distant from zebrafish and has served as an important vertebrate model system in molecular and developmental genetics. We previously isolated a medaka rod opsin gene (R-HI). In the present study we isolated all the cone opsin genes of medaka by genome screening of a lambda-phage and bacterial artificial chromosome (BAC) libraries. The medaka genome contains two red, LWS-A and LWS-B, three green, RH2-A, RH2-B and RH2-C, and two blue, SWS2-A and SWS2-B, subtype opsin genes as well as a single-copy of the ultraviolet, SWS1, opsin gene. Previously only one gene was believed present for each opsin type as reported in a cDNA-based study. These subtype opsin genes are closely linked and must be the products of local gene duplications but not of a genome-wide duplication. Peak absorption spectra (lambda(max)) of the reconstituted photopigments with 11-cis retinal varied greatly among the three green opsins, 452 nm for RH2-A, 5 16 rim for RH2-B and 492 nm for RH2-C, and between the two blue opsins, 439 nm for SWS2-A and 405 nm for SWS2-B. Zebrafish also has multiple opsin subtypes, but phylogenetic analysis revealed that medaka and zebrafish gained the subtype opsins independently. The lambda and BAC DNA clones isolated in this study could be useful for investigating the regulatory mechanisms and evolutionary diversity of fish opsin genes. (c) 2005 Elsevier B.V. All rights reserved.