A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production
A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production
复制标题
棉花丝裂原激活蛋白激酶 (GhMPK6) 参与 ABA 诱导的 CAT1 表达和 H2O2 产生
DOI：
10.1016/j.jgg.2011.10.003
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发表时间：
2011-11-01
影响因子：
5.9
通讯作者：
Li, Xue-Bao
中科院分区：
文献类型：
--
作者：
Luo, Juan;Zhao, Li-Li;Li, Xue-Bao
丝裂原活化蛋白激酶（MAPK）级联是植物中主要的进化保守的信号通路之一，在植物胁迫和发育信号的调控中起着关键作用。在这里，我们鉴定了一个基因，GhMPK6，在棉花中编码MAPK蛋白。GFP荧光分析表明，GhMAPK6是一种细胞质定位蛋白。定量RT-PCR分析显示，脱落酸（ABA）显著促进GhMPK6 mRNA的积累。T-DNA插入突变体atmkk1 （SALK_015914）中GhMPK6基因的过表达使转基因植物对ABA产生了野生型表型。ABA处理显著抑制了GhMPK6转基因系和野生型的子叶绿化/扩张，而atmkk1突变体的子叶绿化/扩张率较高。此外，GhMPK6转基因系和野生型叶片中CAT1表达量和H2O2水平显著高于ABA处理的atmkk1突变体。综上所述，我们的研究结果表明，GhMPK6可能在aba诱导的CAT1表达和H2O2产生中发挥重要作用。
The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays a pivotal role in the regulation of stress and developmental signals in plants. Here, we identified one gene, GhMPK6, encoding an MAPK protein in cotton. GFP fluorescence assay demonstrated that GhMAPK6 is a cytoplasm localized protein. Quantitative RT-PCR analysis revealed that mRNA accumulation of GhMPK6 was significantly promoted by abscisic acid (ABA). Overexpression of GhMPK6 gene in the T-DNA insertion mutant atmkk1 (SALK_015914) conferred a wild-type phenotype to the transgenic plants in response to ABA. Under ABA treatment, cotyledon greening/expansion in GhMPK6 transgenic lines and wild type was significantly inhibited, whereas the atmkk1 mutant showed a relatively high cotyledon greening/expansion ratio. Furthermore, CAT1 expression and H2O2 levels in leaves of GhMPK6 transgenic lines and wild type were remarkably higher than those of atmkk1 mutant with ABA treatment. Collectively, our results suggested that GhMPK6 may play an important role in ABA-induced CAT1 expression and H2O2 production.