FACTORS FAVORING THE ACCUMULATION OF ARABINITOL IN THE YEAST DEBARYOMYCES-HANSENII
FACTORS FAVORING THE ACCUMULATION OF ARABINITOL IN THE YEAST DEBARYOMYCES-HANSENII
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DOI：
10.1139/m85-087
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发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
DACOSTA, MS
中科院分区：
文献类型：
--
作者：
NOBRE, MF;DACOSTA, MS
Culture conditions which lead to the intracellular accumulation of arabinitol were investigated in D. hansenii. Arabinitol, detected in very low concentrations during the exponential phase of growth, accumulated during the stationary phase of growth in yeast extract.sbd.peptone.sbd.1% (wt/vol) glucose medium. This polyol was retained intracellularly even after depletion of exogenous glucose, but was rapidly depleted during regrowth in fresh glucose medium. The accumulation of arabinitol was also favored in media containing 1% (wt/vol) D-fructose, sucrose, L-arabinose, glycerol and sodium acetate. High mannitol levels acumulated in stationary phase cells derived from growth in 1% (wt/vol) D-mannitol and in these cultures only traces of arabinitol were detectable. Intracellular mannitol was also retained after the extracellular mannitol was consumed and was rapidly depleted during regrowth in glucose medium. Arabinitol did not accumulate in basal medium with no added C source, nor in media with nonmetabolizable C sources (D-arabinose or D-ribose). Arabinitol accumulation was independent of the initial glucose concentration between 1% (wt/vol) and about 9% (wt/vol).
