ESTIMATION OF GROWTH FRACTIONS IN MERISTEMS OF ZEA-MAYS-L
ESTIMATION OF GROWTH FRACTIONS IN MERISTEMS OF ZEA-MAYS-L
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DOI：
10.1093/oxfordjournals.aob.a085225
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发表时间：
1976-01-01
期刊：
影响因子：
4.2
通讯作者：
CLOWES, FAL
中科院分区：
文献类型：
--
作者：
CLOWES, FAL
研究了三种估计分生组织群体中循环细胞比例的方法，并在根分生组织中逐个区域进行了研究。只有在静止中心和附近的增殖区域，三种方法产生相似的结果。基于循环细胞的平均循环时间与细胞加倍时间之比的估计是最可靠的。在3h -胸腺嘧啶短脉冲后立即使用标记指数或在长标记周期后立即使用标记指数的两种方法通过计数内复制细胞来夸大生长分数，这些细胞甚至在分生组织的近半层和帽状分生组织的第二层及后续层中也变得突出。讨论了非循环电池的性质。
Three ways of estimating the fraction of cycling cells in a meristematic population were examined region by region throughout the root meristem of Z. mays. Only in the quiescent center and in the nearby proliferating regions did the 3 methods produce similar results. The estimate based on the ratio of the average cycle duration of cycling cells and the cell-doubling time is the most reliable. The 2 methods that use labeling index immediately after a short pulse of 3H-thymidine or after a long labeling period exaggerage the growth fraction by counting endoreduplicating cells which become prominent even in the proximal half of the meristem and in the 2nd and subsequent tiers of the cap meristem. The nature of the non-cycling cells was discussed.