INTERLEUKIN-7 ACTIVATES P56(LCK) AND P59(FYN), 2 TYROSINE KINASES ASSOCIATED WITH THE P90 INTERLEUKIN-7 RECEPTOR IN PRIMARY HUMAN T-CELLS
INTERLEUKIN-7 ACTIVATES P56(LCK) AND P59(FYN), 2 TYROSINE KINASES ASSOCIATED WITH THE P90 INTERLEUKIN-7 RECEPTOR IN PRIMARY HUMAN T-CELLS
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DOI：
10.1002/eji.1830251036
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发表时间：
1995-10-01
影响因子：
5.4
通讯作者：
FOXWELL, BMJ
中科院分区：
文献类型：
--
作者：
PAGE, TH;LALI, FV;FOXWELL, BMJ
我们研究了与克隆的90 kDa（p90）白细胞介素-7受体（IL-7 R）相关的信号传导事件，以确定由该分子引发的信号传导途径的变化是否可以解释T细胞活化后增殖为IL-7的能力。在葡萄激酶测定中使用，我们发现未刺激和活化的人T细胞中的p90 IL-7 R与两种具有内在激酶活性的分子物理相关。蛋白质印迹分析显示这些蛋白质是src激酶，p59（fyn）和p56（lck）。人重组IL-7与p90 IL-7 R的结合导致静息和活化成熟T细胞中两种受体相关激酶的活性增加。因此，通过p90 IL-7 R相关src激酶启动的信号传导途径不太可能仅负责响应于IL-7的仅活化T细胞的增殖。可能来自其他IL-7 R相关分子如γ c的额外信号显然是IL-7驱动的活化原代T细胞增殖所需的。
We have investigated signaling events associated with the cloned 90-kDa (p90) interleukin-7 receptor (IL-7R) to determine whether changes in the signaling pathways initiated by this molecule can explain the ability of T cells to proliferate to IL-7 following activation. Using in vine kinase assays we find that the p90 IL-7R in both unstimulated and activated human T cells is physically associated with two molecules with intrinsic kinase activity. Western blotting analysis reveals these proteins to be the src kinase enzymes, p59(fyn) and p56(lck). Binding of human recombinant IL-7 to the p90 IL-7R results in increased activity of both receptor-associated kinases in both resting and activated mature T cells. Thus, the signaling pathways initiated via the p90 IL-7R-associated src kinases are unlikely to be solely responsible for the proliferation of only activated T cells in response to IL-7. Additional signals, which may derive from other IL-7R-associated molecules such as the gamma c, are clearly required for IL-7-driven proliferation of activated primary T cells.