Quantitative Temporal in Vivo Proteomics Deciphers the Transition of Virus-Driven Myeloid Cells into M2 Macrophages.
Quantitative Temporal in Vivo Proteomics Deciphers the Transition of Virus-Driven Myeloid Cells into M2 Macrophages.
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DOI：
10.1021/acs.jproteome.7b00425
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发表时间：
2017-09-01
影响因子：
4.4
通讯作者：
Gujar S
中科院分区：
文献类型：
--
作者：
Clements DR;Murphy JP;Sterea A;Kennedy BE;Kim Y;Helson E;Almasi S;Holay N;Konda P;Paulo JA;Sharif T;Lee PW;Weekes MP;Gygi SP;Gujar S
骨髓细胞在病毒根除的背景下发挥着核心作用，但这些细胞在整个急性感染过程中如何分化的确切情况却知之甚少。在这项研究中，我们开发了一种新的定量时间体内蛋白质组学（QTiPs）平台，以直接原位捕获时间过渡病毒驱动的骨髓细胞的蛋白质组特征，从而考虑到整个感染过程中的宿主-病毒相互作用。QTiPs与表型、功能和代谢分析相结合，阐明了炎性CD 11b+、Ly 6 G-、Ly 6Chigh-low细胞在抗病毒免疫应答和病毒清除中的关键作用。最重要的是，时间分辨的QTiPs数据集显示了CD 11b+、Ly 6 G-、Ly 6C高-低细胞向M2样巨噬细胞的转变，M2样巨噬细胞在感染后期表现出增加的抗原呈递能力和生物能量需求。我们阐明了骨髓细胞在病毒清除中的关键作用，并显示了这些细胞在表型、功能和代谢方面如何在体内及时从炎症性巨噬细胞转变为M2样巨噬细胞。相对于越来越多的赞赏在体内检查病毒-宿主相互作用和骨髓细胞的作用，本研究阐明了使用定量蛋白质组学揭示的作用和不同的免疫细胞群体的反应，在整个过程中的病毒感染。
Myeloid cells play a central role in the context of viral eradication, yet precisely how these cells differentiate throughout the course of acute infections is poorly understood. In this study, we have developed a novel quantitative temporal in vivo proteomics (QTiPs) platform to capture proteomic signatures of temporally transitioning virus-driven myeloid cells directly in situ, thus taking into consideration host–virus interactions throughout the course of an infection. QTiPs, in combination with phenotypic, functional, and metabolic analyses, elucidated a pivotal role for inflammatory CD11b+, Ly6G–, Ly6Chigh-low cells in antiviral immune response and viral clearance. Most importantly, the time-resolved QTiPs data set showed the transition of CD11b+, Ly6G–, Ly6Chigh-low cells into M2-like macrophages, which displayed increased antigen-presentation capacities and bioenergetic demands late in infection. We elucidated the pivotal role of myeloid cells in virus clearance and show how these cells phenotypically, functionally, and metabolically undergo a timely transition from inflammatory to M2-like macrophages in vivo. With respect to the growing appreciation for in vivo examination of viral–host interactions and for the role of myeloid cells, this study elucidates the use of quantitative proteomics to reveal the role and response of distinct immune cell populations throughout the course of virus infection.
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影响因子：
4.7
作者：
Gujar SA;Lee PW
通讯作者：
Lee PW
影响因子：
3.7
作者：
Boutté AM;McDonald WH;Shyr Y;Yang L;Lin PC
通讯作者：
Lin PC
影响因子：
3.4
作者：
Guo M;Härtlova A;Dill BD;Prescott AR;Gierliński M;Trost M
通讯作者：
Trost M
影响因子：
28.3
作者：
Lachmandas, Ekta;Boutens, Lily;Stienstra, Rinke
通讯作者：
Stienstra, Rinke
DOI：
10.1073/pnas.1525360113
发表时间：
2016-04-19
影响因子：
11.1
作者：
Kloepper, Jonas;Riedemann, Lars;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.