Wholemount in situ hybridization to amphioxus embryos.
Wholemount in situ hybridization to amphioxus embryos.
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DOI：
10.1007/978-1-60327-483-8_48
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发表时间：
2008
影响因子：
--
通讯作者：
P. W. Holland
中科院分区：
文献类型：
--
作者：
P. W. Holland
3. MethodsThe protocol given is designed for amphioxus embryos and larvae, obtained as described in Chapter 38. The method is modified from ref. 1 and has been used to visualize RNA from several developmentally expressed genes of amphi-oxus (see Note 1). With modification, it has also been successfully applied to embryos of other marine invertebrates, notably ascidia (eg, ref. 2).
