Etching of {111} and {210} synthetic pyrite surfaces by two archaeal strains, Metallosphaera sedula and Sulfolobus metallicus
Etching of {111} and {210} synthetic pyrite surfaces by two archaeal strains, Metallosphaera sedula and Sulfolobus metallicus
复制标题
DOI：
10.1016/j.hydromet.2008.05.026
复制
发表时间：
2008-11-01
期刊：
影响因子：
4.7
通讯作者：
Depmeier, Wulf
中科院分区：
文献类型：
--
作者：
Etzel, Katja;Klingl, Andreas;Depmeier, Wulf
为了提高我们对微生物介导的黄铁矿溶解的理解，研究了不同古菌代谢和黄铁矿表面晶体取向的影响。因此，不同方向的合成黄铁矿表面，包括八面体{111}和五边形十二面体{210}面，在长达16天的时间内被Metallosphaera sedula和Sulfolobus Metalus蚀刻。使用铁嗪测定来确定溶液中黄铁矿氧化导致的铁离子浓度。结果表明，景天 M. sedula 在溶液中产生的三价铁比 S. metalus 多，表明景天 M. sedula 是更活跃的生物体。落射荧光显微镜观察表明，两种菌株的细胞部分附着在矿物表面，而其余部分则保持浮游状态，这表明两种生物体可能通过间接生物氧化机制的“接触”和“非接触”机制来溶解黄铁矿。扫描电子显微镜 (SEM) 研究显示整个时间段内细胞附着和蚀刻效应。表面蚀变根据表面晶体学在 16 天的蚀刻中形成尺寸高达 30 μm 的特征，而特征的形状随面对称性而变化。 (C) 2008 Elsevier B.V. 保留所有权利。
To improve our understanding of microbially mediated pyrite dissolution, the influence of different archaeal metabolisms and crystallographic orientations of pyrite surfaces was studied. Therefore, synthetic pyrite surfaces of different orientations, including octahedral {111} and pentagondodecahedral {210} faces, were etched by Metallosphaera sedula and Sulfolobus metallicus during a time period of up to 16 days. A ferrozine assay was used in order to determine the concentration of iron ions in the solution as result of pyrite oxidation. It revealed that M. sedula produces more ferric iron in the solution than S. metallicus, indicating M. sedula as the more active organism. Epifluorescence microscopy observations showed that cells of both strains partly attach to the mineral surface whereas the rest remains planktonic, suggesting that both organisms might act through both, the 'contact' and 'non-contact' mechanisms of the indirect biooxidation mechanisms for the dissolution of pyrite. Studies with Scanning Electron Microscopy (SEM) showed cell attachment and etching effects during the whole time period. Surface alteration forms features depending on surface crystallography up to 30 mu m in size in 16 days of etching, while the shape of the features varies with face-symmetry. (C) 2008 Elsevier B.V. All rights reserved.