Comprehensive Transcriptome Profiles of Streptococcus mutans UA159 Map Core Streptococcal Competence Genes.
Comprehensive Transcriptome Profiles of Streptococcus mutans UA159 Map Core Streptococcal Competence Genes.
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DOI：
10.1128/msystems.00038-15
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发表时间：
2016-03
期刊：
影响因子：
6.4
通讯作者：
Petersen FC
中科院分区：
文献类型：
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作者：
Khan R;Rukke HV;Høvik H;Åmdal HA;Chen T;Morrison DA;Petersen FC
变形链球菌将口腔的坚硬表面作为其自然栖息地，它依靠形成生物膜的能力来生存。对肽信息素响应激活的变形链球菌调节子的全面鉴定为理解变形链球菌如何从个体行为转变为社会行为提供了重要基础。我们的研究将能力期间激活的 29 个转录本中的 27 个置于三个主要调节子内，并揭示了 SigX 调节子内的一组 27 个全链球菌能力激活基因。在变形链球菌（一种与龋齿相关的口腔定植菌）中，自然遗传转化能力的发展是由两种类型的肽信息素触发的，即能力刺激肽 (CSP)（18 个氨基酸 [aa]）或 SigX 诱导肽 (XIP) (7 个氨基酸)。 CSP 诱导的能力是对信息素的晚期反应，需要反应调节因子 ComE 和 XIP 编码基因 comS。 XIP 与 ComR 结合，允许替代 sigma 因子 SigX 及其控制的效应基因的表达。虽然建立了这些监管联系，但每个监管机构控制的一组精确的效应器还没有明确定义。为了改善所有三个调节子的定义，我们使用高分辨率平铺阵列来绘制 CSP 响应早期和晚期基因表达的全局变化。 CSP 反应的早期阶段仅限于与细菌素产生和免疫相关的四个位点的基因表达增加。在后期，上调区域扩展到总共 29 个位点，包括 comS 和 DNA 摄取和重组所需的基因。结果表明，对 CSP 的整个后期反应取决于 comS 的表达，并且由 ComE 介导的对 CSP 的即时转录反应仅限于四个细菌素相关基因座。将新数据与已发表的转录组数据进行比较，可以识别每个调节子中的所有操纵子：ComE 4 个，ComR 2 个，SigX 21 个。最后，通过比较不同链球菌物种的转录组数据，在 SigX 调节子内鉴定了一组 27 个全链球菌能力基因。重要性 变形链球菌将口腔的坚硬表面作为其自然栖息地，它的生存取决于其形成生物膜的能力。对肽信息素响应激活的变形链球菌调节子的全面鉴定为理解变形链球菌如何从个体行为转变为社会行为提供了重要基础。我们的研究将能力期间激活的 29 个转录本中的 27 个置于三个主要调节子内，并揭示了 SigX 调节子内的一组 27 个全链球菌能力激活基因。
S. mutans has the hard surfaces of the oral cavity as its natural habitat, where it depends on its ability to form biofilms in order to survive. The comprehensive identification of S. mutans regulons activated in response to peptide pheromones provides an important basis for understanding how S. mutans can transition from individual to social behavior. Our study placed 27 of the 29 transcripts activated during competence within three major regulons and revealed a core set of 27 panstreptococcal competence-activated genes within the SigX regulon. In Streptococcus mutans, an oral colonizer associated with dental caries, development of competence for natural genetic transformation is triggered by either of two types of peptide pheromones, competence-stimulating peptides (CSPs) (18 amino acids [aa]) or SigX-inducing peptides (XIPs) (7 aa). Competence induced by CSP is a late response to the pheromone that requires the response regulator ComE and the XIP-encoding gene comS. XIP binds to ComR to allow expression of the alternative sigma factor SigX and the effector genes it controls. While these regulatory links are established, the precise set of effectors controlled by each regulator is poorly defined. To improve the definition of all three regulons, we used a high-resolution tiling array to map global changes in gene expression in the early and late phases of the CSP response. The early phase of the CSP response was limited to increased gene expression at four loci associated with bacteriocin production and immunity. In the late phase, upregulated regions expanded to a total of 29 loci, including comS and genes required for DNA uptake and recombination. The results indicate that the entire late response to CSP depends on the expression of comS and that the immediate transcriptional response to CSP, mediated by ComE, is restricted to just four bacteriocin-related loci. Comparison of the new data with published transcriptome data permitted the identification of all of the operons in each regulon: 4 for ComE, 2 for ComR, and 21 for SigX. Finally, a core set of 27 panstreptococcal competence genes was identified within the SigX regulon by comparison of transcriptome data from diverse streptococcal species. IMPORTANCE S. mutans has the hard surfaces of the oral cavity as its natural habitat, where it depends on its ability to form biofilms in order to survive. The comprehensive identification of S. mutans regulons activated in response to peptide pheromones provides an important basis for understanding how S. mutans can transition from individual to social behavior. Our study placed 27 of the 29 transcripts activated during competence within three major regulons and revealed a core set of 27 panstreptococcal competence-activated genes within the SigX regulon.
链特异性的转录组分析，在基因组平铺微阵列上直接标记RNA。
影响因子：
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作者：
Yu WH;Høvik H;Olsen I;Chen T
通讯作者：
Chen T