Intracellular coupling modulates biflagellar synchrony
Intracellular coupling modulates biflagellar synchrony
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DOI：
10.1098/rsif.2020.0660
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发表时间：
2021-01-27
影响因子：
3.9
通讯作者：
Kanso, Eva
中科院分区：
文献类型：
--
作者：
Guo, Hanliang;Man, Yi;Kanso, Eva
鞭毛跳动表现出多种同步模式。这种同步性长期以来一直被归因于鞭毛之间的流体动力耦合。然而，最近对鞭毛藻的研究表明，细胞内部的一种机制，通过连接鞭毛基本体的收缩纤维，必须发挥作用，以主动调节鞭毛的同步性。到底基偶联是如何调节鞭毛协调的还不清楚。在这里，我们使用一系列复杂程度递减的数学模型来检验基础耦合在双鞭毛虫衣藻同步过程中的作用。我们报道，即使在没有流体动力耦合和鞭毛顺应性的情况下，基础耦合也足以实现同相、反相和双稳态同步。这些模式可以通过调节单个鞭毛的活动水平或基础耦合的强度来实现。当允许不同的鞭毛活动时，我们观察到滑移模式，就像在活细胞实验中一样。我们引入了一个无量纲的鞭毛活动与基偶联的比率，它可以预测同步模式。这一比率使我们能够查询尚不能通过实验直接测量的生物参数。我们的工作为细胞主动控制鞭毛的同步化提供了一条具体的途径。
Beating flagella exhibit a variety of synchronization modes. This synchrony has long been attributed to hydrodynamic coupling between the flagella. However, recent work with flagellated algae indicates that a mechanism internal to the cell, through the contractile fibres connecting the flagella basal bodies, must be at play to actively modulate flagellar synchrony. Exactly how basal coupling mediates flagellar coordination remains unclear. Here, we examine the role of basal coupling in the synchronization of the model biflagellate Chlamydomonas reinhardtii using a series of mathematical models of decreasing levels of complexity. We report that basal coupling is sufficient to achieve inphase, antiphase and bistable synchrony, even in the absence of hydrodynamic coupling and flagellar compliance. These modes can be reached by modulating the activity level of the individual flagella or the strength of the basal coupling. We observe a slip mode when allowing for differential flagellar activity, just as in experiments with live cells. We introduce a dimensionless ratio of flagellar activity to basal coupling that is predictive of the mode of synchrony. This ratio allows us to query biological parameters which are not yet directly measurable experimentally. Our work shows a concrete route for cells to actively control the synchronization of their flagella.