SENSITIVITY OF BIOFILMS TO ANTIMICROBIAL AGENTS
SENSITIVITY OF BIOFILMS TO ANTIMICROBIAL AGENTS
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DOI：
10.1111/j.1365-2672.1993.tb04345.x
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
GILBERT, P
中科院分区：
文献类型：
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作者：
BROWN, MRW;GILBERT, P
通常，在自然界中，微生物与固体表面密切相关，无论这些表面是软组织的、活体的，还是与管道、淹没材料或土壤中的颗粒相关的营养惰性表面(Costerton等人。1987)。与固体表面的缔合导致微生物生物膜的产生。这些可以被认为是在广泛的胞外聚合物糖环内组织的生物体联合体(Costerton等人。(1981年)。虽然在体内生物膜通常代表单一培养物(例如，留置医疗设备的表皮葡萄球菌感染)，但在其他情况下，它们更有可能包括许多不同的物种，不仅代表细菌，还代表藻类和原生动物。1985年)。这些措施包括对体内宿主免疫防御的保护，以及为在其他地方的生长和生存提供和维持适当的物理化学环境。在后一种情况下，虽然糖萼可以起到离子交换柱的作用，并排除大的、高电荷的分子，但大多数溶质将在其上平衡并进入常住人口。糖基化的扩散限制和局部的高密度-
Often, in nature, micro-organisms grow in close association with solid surfaces whether these be of soft tissues, in vivo, or on nutritionally inert surfaces associated with pipework, submerged materials or particulates in soil (Costerton et al. 1987). Associations with solid surfaces lead to the generation of microbial biofilms. These may be regarded as consortia of organisms organized within extensive exopolymer glycocalices (Costerton et al. 1981). Whilst in vivo biofilms often represent mono-cultures (eg Staphylococcus epidermidis infections of indwelling medical devices), in other situations they are more likely to comprise many diverse species representing not only bacteria but also algae and protozoa.Organization as biofilms is thought to confer many advantages upon the component organisms (Costerton et al. 1985). These include protection from host immune defences in vivo, and provision and maintenance of appropriate physico-chemical environments for growth and survival elsewhere. In this latter respect, whilst the glycocalyx can function as an ion-exchange column and exclude large, highly charged molecules, most solutes will equilibrate across it and access the resident population. Diffusion limitation by the glycocalyx together with localized high den-