Immune cell infiltration landscapes in pediatric acute myocarditis analyzed by CIBERSORT
Immune cell infiltration landscapes in pediatric acute myocarditis analyzed by CIBERSORT
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DOI：
10.1016/j.jjcc.2020.08.004
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发表时间：
2021-02-01
影响因子：
2.5
通讯作者：
Ito, Yoshinori
中科院分区：
文献类型：
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作者：
Kawada, Jun-ichi;Takeuchi, Suguru;Ito, Yoshinori
背景：心肌炎是一种心肌炎性疾病，可导致心功能不全和心力衰竭。以往的研究表明，先天免疫和适应性免疫之间复杂的相互作用参与了急性心肌炎的发病机制。免疫组织化学是目前评估浸润性免疫细胞的标准方法，然而，使用这种技术很难研究和量化许多免疫细胞群体。通过估计RNA转录物的相对亚群，对5例心肌炎患儿的心肌内膜活检样本进行细胞类型鉴定（CIBERSORT），一种用于从组织基因表达谱定量细胞级分的计算方法。CIBERSORT结果，然后与免疫组化analysis.Results：免疫浸润反卷积的显着结果，获得了4例暴发性心肌炎的CIBERSORT分析。在22种免疫细胞类型中，在一个或多个患者中检测到19种细胞类型。活化的NK细胞是2例患者中最常见的细胞群，而活化的记忆性CD4（+）T细胞和M2巨噬细胞是各1例患者中最常见的细胞群。总体CIBERSORT结果与免疫组化结果一致，虽然有些差异observed.Conclusions：浸润免疫细胞亚群检测CIBERSORT分析可以反映先天性和适应性免疫反应在急性心肌炎的时间进程。CIBERSORT可能具有表征心肌组织中浸润免疫细胞的细节的潜力，并为急性心肌炎的发病机制提供新的见解。(C)2020年由Elsevier Ltd代表日本心脏病学院出版。
Background: Myocarditis is an inflammatory disease of the myocardium, which leads to cardiac dysfunction and heart failure. Previous studies have suggested that complex cross-talk between innate and adaptive immune responses is involved in the pathogenesis of acute myocarditis. Immunohistochemistry is the current standard method for the evaluation of infiltrating immune cells, however, it is difficult to investigate and quantify many immune cell populations using this technique.Methods: Endomyocardial biopsy samples of five pediatric patients with myocarditis were analyzed by cell-type identification by estimating relative subsets of RNA transcript (CIBERSORT), a computational method for quantifying cell fractions from tissue gene expression profiles. CIBERSORT results were then compared with immunohistochemistry analyses.Results: Significant results of immune infiltrate deconvolution were obtained in four patients with fulminant myocarditis by CIBERSORT analysis. Among 22 immune cell types, 19 cell types were detected in one or more patients. Activated NK cells were the most prevalent population in two patients, whereas activated memory CD4(+) T cells and M2 macrophages were the most prevalent population in one patient each. Overall CIBERSORT results were consistent with those of immunohistochemistry, although some discrepancies were observed.Conclusions: Infiltrating immune cell subsets detected by CIBERSORT analysis can reflect the time course of innate and adaptive immune responses in acute myocarditis. CIBERSORT may have the potential to characterize the detail of infiltrating immune cells in myocardial tissues and provide novel insights into the pathogenesis of acute myocarditis. (C) 2020 Published by Elsevier Ltd on behalf of Japanese College of Cardiology.