Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H-pylori by an immunomagnetic-bead separation technique
Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H-pylori by an immunomagnetic-bead separation technique
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DOI：
10.1128/jcm.36.1.321-323.1998
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发表时间：
1998-01-01
影响因子：
9.4
通讯作者：
Kamiya, S
中科院分区：
文献类型：
--
作者：
Osaki, T;Taguchi, H;Kamiya, S
通过免疫磁珠（IMB）分离技术，尝试从感染幽门螺杆菌的无菌小鼠的胃肠道和粪便样本中分离出幽门螺杆菌。 IMB分离后胃样品中H. pylori的分离率不高于直接培养样品。 IMB 分离粪便后，通过 PCR 可检测到幽门螺杆菌，尽管幽门螺杆菌不可培养。
By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pylori was detectable by PCR, although H. pylori was not culturable.