Effects of urate, a natural inhibitor of peroxynitrite-mediated toxicity, in the response of Arabidopsis thaliana to the bacterial pathogen Pseudomonas syringae
Effects of urate, a natural inhibitor of peroxynitrite-mediated toxicity, in the response of Arabidopsis thaliana to the bacterial pathogen Pseudomonas syringae
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DOI：
10.1046/j.1365-313x.2001.00984.x
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发表时间：
2001-03-01
期刊：
影响因子：
7.2
通讯作者：
García-Olmedo, F
中科院分区：
文献类型：
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作者：
Alamillo, JM;García-Olmedo, F
尿酸盐是一种天然的过氧亚硝酸盐清除剂，已被用来研究过氧亚硝酸盐在植物与病原体相互作用中的可能作用。尿酸盐大大减少了用非生物过氧亚硝酸盐生成系统或过氧亚硝酸盐溶液处理的拟南芥叶片中病斑的形成，表明它可以作为植物中的有效清除剂。在与无毒力的丁香假单胞菌的相互作用中。菜豆 (avrRPM1(+)) 中，尿酸盐可显着减少接种区域的细胞死亡，而不会影响抗病性。相比之下，尿酸盐促进了对同基因丁香假单胞菌 (avrRPM1(-)) 的离散细胞死亡，单独接种时不会触发 HR，但它会诱导抵抗和病原体生长的抑制。过氧亚硝酸盐的清除并没有改变拟南芥对野油菜黄单胞菌 pv 油菜无毒菌株的反应，该菌株对 NO 和过氧亚硝酸盐表现出高抗性。我们的数据表明，过氧亚硝酸盐在植物对丁香假单胞菌的反应中发挥着重要作用。
Urate, a natural peroxynitrite scavenger, has been used to investigate the possible role of peroxynitrite during plant-pathogen interactions. Urate greatly reduced lesion formation in Arabidopsis leaves treated with an abiotic peroxynitrite-generating system or with a peroxynitrite solution, indicating that it can act as an effective scavenger in planta. In the interaction with the avirulent Pseudomonas syringae pv. phaseolicola (avrRPM1(+)), cell death in the inoculated area was strongly reduced by urate, without compromising disease resistance. In contrast, urate promoted discrete cell death in response to an isogenic Pseudomonas syringae (avrRPM1(-)), which did not trigger an HR when inoculated alone, and it induced resistance and arrest of pathogen growth. Scavenging of peroxynitrite did not modify the response of Arabidopsis to an avirulent strain of Xanthomonas campestris pv campestris, that showed a high resistance to NO and peroxynitrite. Our data indicate that peroxynitrite plays a significant role in the responses of plants to Pseudomonas syringae.