Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively
Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively
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表达咔唑 1,9a-双加氧酶的重组热带根瘤菌菌株对二恶英类化合物的根修复
DOI：
10.1271/bbb.67.1144
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
T. Omori
中科院分区：
文献类型：
--
作者：
Yuko Saiki;H. Habe;T. Yuuki;M. Ikeda;T. Yoshida;H. Nojiri;T. Omori
重组根瘤菌PBK3-IS是一株结构性表达鞘氨醇单胞菌咔唑1，9a-双加氧酶组分的重组根瘤菌。构建了菌株KA1。在接种菌株pBK3-IS的水培西里特罗根际中，初始底物为25μg时，3d内二苯并呋喃的降解率为48%。在使用无菌蛭石的土壤培养的西里特罗根际也得到了类似的结果。用非灭菌的田间土壤代替灭菌的蛭石，接种的重组菌在除湿润稻田外的所有供试土壤上均能在无菌的根上生长。
A recombinant Rhizobium strain, PBK3-IS, that constitutively expressed the oxygenase component of carbazole 1,9a-dioxygenase from Sphingomonas sp. strain KA1, was constructed. In the water-cultured siratro rhizospheres inoculated with strain PBK3-IS, 48% of the dibenzofuran was removed within 3 days (initial substrate, 25 μg). Similar results were obtained in soil-cultured siratro rhizospheres using sterile vermiculite. When non-sterile field soils were used instead of sterile vermiculite, the inoculated recombinant strain could grow on the siratro root in all soils tested, except for wet paddy field.
影响因子：
2.6
作者：
ELZER, PH;KOVACH, ME;ROOF, RM
通讯作者：
ROOF, RM