The release of sex-specific substances responsible for sexual agglutination from haploid cells of Saccharomyces cerevisiae.
The release of sex-specific substances responsible for sexual agglutination from haploid cells of Saccharomyces cerevisiae.
复制标题
酿酒酵母单倍体细胞释放负责性凝集的性别特异性物质。
DOI：
10.1016/0014-4827(77)90090-8
复制
发表时间：
1977
影响因子：
3.7
通讯作者：
N. Yanagishima
中科院分区：
文献类型：
--
作者：
M. Hagiya;K. Yoshida;N. Yanagishima
用一种新建立的高压灭菌方法，从酿酒酵母异源单倍体细胞中成功地大量释放了负责有性细胞凝集的细胞表面物质。考察了产生这种释放现象的条件。将细胞悬浮在30 mM Tris-HCl、pH 7.0、5 mM EDTA溶液中，在120℃、压力为1 kg/cm2的压力下灭菌3min，对性凝集物质的增溶效果最好。这些物质被相反交配型的细胞表面特异性地吸附，导致相反交配型的细胞的凝集性被掩盖。这些物质没有从缺乏性细胞凝集的细胞表面释放。还提出了一些证据，表明在体外凝集物质和α凝集物质之间形成了分子复合体。上述方法适用于不同菌株的表面凝集物质的增溶。酿酒。
Cell surface substances responsible for sexual cell agglutination were successfully released in a large quantity from heterothallic haploid cells ofSaccharomyces cerevisiaeby a newly established autoclaving method. The conditions for this releasing phenomenon were examined. The sexual agglutination substances were solubilized most efficiently when the cells, suspended in a 30 mM Tris-HCl, pH 7.0, 5 mM EDTA solution, were autoclaved at a pressure of 1 kg/cm2at 120 °C for 3 min. The substances were specifically adsorbed by the cell surface of the opposite mating type, resulting in the masking of agglutinability of the cells of the opposite mating type. The substances were not released from the surface of cells which lacked sexual cell agglutination. The evidence suggesting the formation of a molecular complex betweena- and α-agglutination substances in vitro is also presented. The above procedure is applicable to the solubilization of surfage agglutination substances from various strains ofS. cerevisiae.